Identification of specific molecular markers linked to the rust resistance gene M4 in flax
文献类型： 外文期刊
作者： Bo, T. Y. 1 ; Ma, J. J. 1 ; Chen, J. X. 1 ; Miao, T. Y. 1 ; Zhai, W. X. 3 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201106, Peoples R China
2.Shanghai Key Lab Protected Hort Technol, Shanghai 201106, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
期刊名称：AUSTRALASIAN PLANT PATHOLOGY （ 影响因子：1.599； 五年影响因子：1.708 ）
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收录情况： SCI
摘要： To identify molecular markers linked to the flax rust-resistance gene M4, RAPD analysis of NM4 (a near-isogenic line containing the M4 gene) and the recurrent parent Bison was performed using 540 decamer primers. The primer OPA18 amplified a specific fragment, OPA18, in the NM4 line. The OPA18 marker was found to be closely linked to the M4 gene, with a genetic distance of 2.1 cM, through the analysis of the F segregating population derived from a cross of Bison x NM4. Based on the sequence of OPA18, the specific PCR primers were designed and a SCAR marker for the M4 gene was developed. The amplification of different resistant germplasm proved that the marker is specific for the M4 gene. This marker has been used successfully in marker-assisted selection in the flax breeding program.
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