Isolation and characterization of Methanoculleus receptaculi sp nov from Shengli oil field, China
文献类型: 外文期刊
作者: Cheng, Lei 1 ; Qiu, Tian-Lei 1 ; Li, Xia 1 ; Wang, Wei-Dong 3 ; Deng, Yu 1 ; Yin, Xiao-Bo 1 ; Zhang, Hui 1 ;
作者机构: 1.Biogas Inst Minist Agr, Sect 4 13, Chengdu 610041, Sichuan, Peoples R China
2.Beijing Agrobiotechnol Res Ctr, Beijing, Peoples R China
3.SinoPEC, Shengli Oil Field Co Ltd, Oil Prod Technol Res Inst, Dongying, Peoples R China
关键词: Methanoculleus receptaculi;oil reservoir;acetoclastic methanogenesis;hydrogenotrophic methanogenesis;METHANOGENIC BACTERIA;PHYLOGENETIC DIVERSITY;METHANOLOBUS-SICILIAE;PETROLEUM RESERVOIRS;GEN. NOV.;WATER;EMENDATION;OLENTANGYI;WELL;BIODEGRADATION
期刊名称:FEMS MICROBIOLOGY LETTERS ( 影响因子:2.742; 五年影响因子:2.856 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Three strictly anaerobic, thermophilic methanogens (ZC-2(T), ZC-3 and ZC-6) were isolated from Shengli oil field, China. The 16S rRNA gene sequences of the three strains were nearly identical, possessing > 99.8% sequence similarity. They also possessed high sequence similarity, 97.4%, to Methanoculleus palmolei strain INSLUZ(T) (97.4% and 97.5%, respectively), indicating that they represented a novel species within the genus Methanoculleus. Cells of strain ZC-2(T) were nonmotile cocci, 0.8-1.7 mu m in diameter, and always occurred singly or in pairs. The three strains used H-2/CO2 or sodium formate as substrates for methanogenesis but not sodium acetate, trimethylamine, monomethylamine, ethanol, dimethyl sulfide, isopropanol, isobutanol, butan-2-ol or H-2/CO. Optimum growth of strain ZC-2(T) occurred in the presence of 0.2 M NaCl, pH 7.5-7.8 and temperature 50-55 degrees C with a specific growth rate of 0.084 h(-1). The mol% G+C content of the genomic DNA was 55.2 mol%. Based on these phenotypic and phylogenetic characteristics, strains ZC-2(T), ZC-3 and ZC-6 are proposed to represent a novel species in the genus Methanoculleus and named Methanoculleus receptaculi sp. nov. The type strain is ZC-2(T) (CGMCC 1.5087(T)=DSM 18860(T)).
- 相关文献
作者其他论文 更多>>
-
PHYTOTOXITY OF POLYCYCLIC AROMATIC HYDROCARBONS TO SALIX VIMINALISL
作者:Li, Xia;Chen, Fengzhen;Liu, Junxiang;Wang, Yuancheng;Li, Ao;Sun, Zhenyuan;Cheng, Yunhe;Zhai, Feifei
关键词:Gene expression; PAH stress; Physiological change; Phytotoxicity
-
Octoploids Show Enhanced Salt Tolerance through Chromosome Doubling in Switchgrass (Panicum virgatum L.)
作者:Ye, Jiali;Fan, Yupu;Zhang, Hui;Teng, Wenjun;Teng, Ke;Wu, Juying;Fan, Xifeng;Yue, Yuesen;Ye, Jiali;Fan, Yupu;Wang, Shiwen;Fan, Yupu;Wang, Shiwen
关键词:Panicum virgatum L.; octoploid; salt stress; transcriptome; WGCNA
-
International Development Trends in the Field of Agricultural Resources and the Environment
作者:Chuan, Limin;Qi, Shijie;Zhang, Hui;Jia, Qian;Wang, Ailing;Zhao, Jingjuan
关键词:agriculture; resources; environment; research topics; development trends
-
Research Frontiers in the Field of Agricultural Resources and the Environment
作者:Chuan, Limin;Zhao, Jingjuan;Qi, Shijie;Jia, Qian;Zhang, Hui;Ye, Sa
关键词:LDA model; research frontier; agricultural resources and the environment; frontier indicators; theme fusion
-
Spectral Detection of Peanut Southern Blight Severity Based on Continuous Wavelet Transform and Machine Learning
作者:Guo, Wei;Sun, Heguang;Qiao, Hongbo;Zhang, Hui;Dong, Ping;Sun, Heguang;Song, Xiaoyu;Zhou, Lin
关键词:peanut southern blight; reflection spectrum; spectral index; continuous wavelet transform; machine learning
-
The first complete mitochondrial genome of Carex (C. breviculmis): a significantly expanded genome with highly structural variations
作者:Xu, Shenjian;Teng, Ke;Zhang, Hui;Wu, Juying;Zhang, Hongyu;Wen, Haifeng;Teng, Wenjun;Yue, Yuesen;Fan, Xifeng;Duan, Liusheng
关键词:Genome recombination; Mitochondrial genome; Organelle genome; RNA editing; Carex
-
Progress in the Application of CNN-Based Image Classification and Recognition in Whole Crop Growth Cycles
作者:Yu, Feng;Xiao, Jun;Yu, Feng;Zhang, Qian;Ma, Yuntao;Wang, Ming;Luan, Rupeng;Liu, Xin;Ping, Yang;Nie, Ying;Tao, Zhenyu;Zhang, Hui
关键词:convolution neural network; crop classification; crop recognition; whole crop growth cycle