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Isolation and characterization of Methanoculleus receptaculi sp nov from Shengli oil field, China

文献类型: 外文期刊

作者: Cheng, Lei 1 ; Qiu, Tian-Lei 1 ; Li, Xia 1 ; Wang, Wei-Dong 3 ; Deng, Yu 1 ; Yin, Xiao-Bo 1 ; Zhang, Hui 1 ;

作者机构: 1.Biogas Inst Minist Agr, Sect 4 13, Chengdu 610041, Sichuan, Peoples R China

2.Beijing Agrobiotechnol Res Ctr, Beijing, Peoples R China

3.SinoPEC, Shengli Oil Field Co Ltd, Oil Prod Technol Res Inst, Dongying, Peoples R China

关键词: Methanoculleus receptaculi;oil reservoir;acetoclastic methanogenesis;hydrogenotrophic methanogenesis;METHANOGENIC BACTERIA;PHYLOGENETIC DIVERSITY;METHANOLOBUS-SICILIAE;PETROLEUM RESERVOIRS;GEN. NOV.;WATER;EMENDATION;OLENTANGYI;WELL;BIODEGRADATION

期刊名称:FEMS MICROBIOLOGY LETTERS ( 影响因子:2.742; 五年影响因子:2.856 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Three strictly anaerobic, thermophilic methanogens (ZC-2(T), ZC-3 and ZC-6) were isolated from Shengli oil field, China. The 16S rRNA gene sequences of the three strains were nearly identical, possessing > 99.8% sequence similarity. They also possessed high sequence similarity, 97.4%, to Methanoculleus palmolei strain INSLUZ(T) (97.4% and 97.5%, respectively), indicating that they represented a novel species within the genus Methanoculleus. Cells of strain ZC-2(T) were nonmotile cocci, 0.8-1.7 mu m in diameter, and always occurred singly or in pairs. The three strains used H-2/CO2 or sodium formate as substrates for methanogenesis but not sodium acetate, trimethylamine, monomethylamine, ethanol, dimethyl sulfide, isopropanol, isobutanol, butan-2-ol or H-2/CO. Optimum growth of strain ZC-2(T) occurred in the presence of 0.2 M NaCl, pH 7.5-7.8 and temperature 50-55 degrees C with a specific growth rate of 0.084 h(-1). The mol% G+C content of the genomic DNA was 55.2 mol%. Based on these phenotypic and phylogenetic characteristics, strains ZC-2(T), ZC-3 and ZC-6 are proposed to represent a novel species in the genus Methanoculleus and named Methanoculleus receptaculi sp. nov. The type strain is ZC-2(T) (CGMCC 1.5087(T)=DSM 18860(T)).

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