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Analysis and Characterization of Expressed Sequence Tag (EST) and Simple Sequence Repeats (SSR) from Red Sea Bream, Chrysophrys major, and Gilthead Sea Bream, Sparus aurata

文献类型： 外文期刊

作者： Liu, Yun-Guo 1 ; Chen, Song-Lin 3 ; Liu, Chun-Ying 4 ; Li, Fang-Zheng 4 ;

作者机构： 1.Ocean Univ China, Fishery Coll, Qingdao 266003, Peoples R China

2.Shandong Entry Exit Inspect & Quarantine Bur, Agr Prod Inspect Ctr, Qingdao 266002, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

4.Qingdao Agr Univ, Qingdao 266109, Peoples R China

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.287； 五年影响因子：0.593 ）

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收录情况： SCI

摘要： A total of 101 and 119 simple sequence repeats (SSR) were obtained from expressed sequence tags (EST) of the red sea bream (Chrysophrys major) and gilthead sea bream (Sparus aurata), respectively. Dimeric repeats were the most abundant class of SSR in both red (70.30%) and gilthead (51.26%) sea bream. Trimeric, tetrameric, and pentameric repeats occurred in decreasing proportions, i.e., 21.36%, 6.8%, and 0.97% in red sea bream and 45.38%, 3.36%, and 0 in gilthead. There were no hexameric repeats in EST of either species. The frequency of every class of SSR (dimeric, trimeric, tetrameric, and pentameric) decreased with increasing repeat length. In cross-species amplification of 20 existing microsatellite loci from gilthead sea bream in 20 red sea bream specimens, twelve loci showed positive amplification and four loci showed polymorphic amplification with two or three alleles.

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