文献类型： 外文期刊

作者： Qian, Y ¹ ; Shi, WQ ² ; Ding, JT ² ; Sha, JH ² ; Fan, BQ ² ;

作者机构： 1.Nanjing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Human Clin Reprod Med Ctr, Nanjing 210029, Peoples R China

2.Nanjing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Human Clin Reprod Med Ctr, Nanjing 210029, Peoples R China; Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China; Yangzhou Univ, Coll Anim Sci & Vet Med, Yangzhou 225009, Peoples R China; Nanjing Med Univ, Jiangsu Province Key Lab Reprod Med, Nanjing 210029, Peoples R China

关键词： Porcine;Cumulus-oocytes complexes;In vitro maturation;Cumulus;Bovine follicular-fluid;Male pronucleus formation;In-vitro;Invitro;fertilization;Pig oocytes;Subsequent development;Meiotic resumption;Granulosa-cells;Hyaluronic-acid;Mouse oocytes

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

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收录情况： SCI

摘要： The objective of the present study was to investigate the correlation between the degree of cumulus expansion and in vitro development of porcine cumulus-oocytes complexes (COCs) matured and fertilized in vitro. The COCs were matured in the maturation medium (IVMM) supplemented with 15% or 5% of porcine follicular fluid (PFF) from small, medium and large follicles (<2 mm, 2-5 mm and >5 mm, respectively). COCs cultured in IVMM with PFF for 48 h displayed less expansion than those cultured in IVMM alone (P<0.05), irrespective of follicle size. After culture for 24 h in IVMM with PFF and for another 24 h in IVMM alone, the degree of cumulus expansion was more prominent than culture in the presence of PFF for the entire 48 h period (P<0.05), but the percentages of oocytes with PB I showed no significant difference between the control and experimental groups (P>0.05). After in vitro fertilization, the oocytes failed to develop to the morula/blastocyst stages except for those matured in IVMM supplemented with 15% or 5% PFF obtained from >5 mm follicles for the first 24 h and followed by in IVMM alone for the second 24 h (12.5% and 11.1% of the embryos developed to morulae and blastocysts, respectively). The expanded cumulus areas of COCs were significantly positively correlated with their in vitro development (p=0.0058, 0.0001 and 0.0348 for the percentages of embryos developed to 2-4 cell, beyond 4 cell and morula and blastocyst stages, respectively). In conclusion, PFF had an inhibiting effect on cumulus expansion, and the inhibitory effect decreased progressively with the increase in size of follicles from which PFF was obtained, and the action of PFF on cumulus expansion was affected by the PFF culture time. The areas of the expanded cumulus mass may be used as a parameter to predict development of porcine oocytes matured and fertilized in vitro.