Modeling of the partitioning of membrane protein and phase equilibria for Triton X-100-salt aqueous two-phase systems using a modified generalized multicomponent osmotic virial equation
文献类型： 外文期刊
作者： Xie, HG 1 ; Wang, YJ 2 ; Sun, M 2 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
关键词： aqueous two-phase system (ATPS);surfactant;partition coefficient;phase equilibria;membrane protein
期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）
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收录情况： SCI
摘要： The partitioning of membrane protein and liquid-liquid equilibria for Triton X-100-salt-water aqueous two-phase systems were measured at 293.15 K. A generalized model was proposed to predict both partitioning of membrane protein and phase behavior of the surfactant aqueous two-phase systems. By using part of liquid-liquid equilibria (LLE) data, Triton X-100-Na_2SO_4 and Triton X-100-Na_2CO_3 aqueous two-phase systems were successfully predicted. The results showed that most of the average absolute deviations were less than 1.0% (w/w). The partition coefficient of membrane protein in Triton X-100-Na_2SO_4 and Triton X-100-Na_2CO_3 aqueous two-phase systems were also successfully predicted. The results showed that most of the average absolute deviations of the two systems were less than 5% and 8%, respectively. The model could give good representation of partitioning of membrane protein and the phase diagrams.
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