您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library

文献类型： 外文期刊

作者： Wang, Hongjun 1 ; Gao, Yaping 2 ; Gong, Yumei 2 ; Chen, Xiaoling 3 ; Liu, Chuan; Zhou, Xuemei; Blackall, P. J.;

作者机构： 1.China Agr Univ, Coll Vet Med, Minist Agr, Key Lab Prevent Vet Med, Beijing 100094, Peoples R China

2.China Agr Univ, Coll Vet Med, Minist Agr, Key Lab Prevent Vet Med, Beijing 100094, Peoples R China; Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100089, Peoples R China; Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China; Dept Primary Ind & Fisheries, Anim Res Inst, Yeerongpilly, Qld 4105, Australia

3.China Agr Univ, Coll Vet Med, Minist Agr, Key Lab Prevent Vet Med, Beijing 100094, Peoples R China; Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100089, Peoples R China; Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China; Dept

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Avibacterium paragallinarum is the causative agent of infectious coryza. The protective antigens of this important pathogen have not yet been clearly identified. In this paper, we applied phage display technique to screen the immunodominant mimotopes of a serovar A strain of A. paragallinarum by using a random 12-peptide library, and evaluated the immunogenicity in chickens of the selected mimotope. Polyclonal antibody directed against A. paragallinarum strain 0083 (serovar A) was used as the target antibody and phage clones binding to this target were screened from the 12-mer random peptide library. More than 50% of the phage clones selected in the third round carried the consensus peptide motif sequence A-DP(M)L. The phage clones containing the peptide motif reacted with the target antibody and this interaction could be blocked, in a dose-dependent manner, by A. paragallinarum. One of the peptide sequences, YGLLAVDPLFKP, was selected and the corresponding oligonucleotide sequence was synthesized and then inserted into the expression vector pFliTrx. The recombinant plasmid was transferred into an expression host Escherichia coli GI826 by electroporation, resulting in a recombinant E. coli expressing the peptide on the bacterial surface. Intramuscular injection of the epitope-expressing recombinant bacteria into chickens induced a specific serological response to serovar A. A. paragallinarum. The chickens given the recombinant E. coli showed significant protection against challenge with A. paragallinarum 0083. These results indicated a potential for the use of the mimotope in the development of molecular vaccines for infectious coryza.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台