文献类型： 外文期刊

作者： Jia, CH ¹ ; Zhao, HY ² ; Wang, HZ ² ; Xing, ZF ² ; Du, KJ ² ; Song, YR ² ; Wei, JH ² ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China; Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China

关键词： triploid Chinese white poplar;4CL;lignin;holocellulose;Wiesner reaction

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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收录情况： SCI

摘要： The antisense 4CL (4-coumarate: CoA ligase) gene was transformed into triploid Chinese white poplar (Populus tomentosa) mediated by Agrobacterium tumefaciens. PCR and Southern blot analysis indicated that antisense 4CL gene had been integrated into the genome of the transgenic Chinese white poplars. The antisense gene had also been expressed, which was indicated by RT-PCR and Western analysis. Klason lignin content assay showed that repression of 4CL expression could result in remarkable +reduction of lignin content in transgenic poplars, with most reduction of 41.73 percent compared with that of wild type in this paper. But there is no significant difference in holocellulose content between trans-genic and wild poplars. We considered that 4CL might not be the metabolism control point between lignin and carbohy-drate biosynthesis. The stems of transgenic poplars displayed red-brown color with different levels after the bark was peeled, while those of untransformed plants were white. No visible differences in growth and development were observed between transgenic and wild plants. Wiesner reaction analysis of the transgenic plant stems with reduced lignin content exhibited red color, while that of untransformed plant was typically purple-red.