文献类型： 外文期刊
作者： Wang, HZ 1 ; Ma, RC 2 ; Li, RF 2 ; Wang, GY 2 ; Wei, JH 2 ;
作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 10094, Peoples R China
2.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 10094, Peoples R China; Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China
关键词： DHS;Nicotiana benthamiana;VIGS;senescence;promoting growth
期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）
ISSN：
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收录情况： SCI
摘要： A cDNA fragment corresponding to deoxyhypusine synthase gene NbDHS was isolated and cloned into potato virus X (PVX) vector for functional analysis in Nicotiana benthamiana by using virus-induced gene silencing (VIGS). Plants agroinfected with recombinant virus vector PVX-NbDHS exhibited an increase in leaf biomass, delay in natural leaf senescence and flowering time, and decrease in leaf chlorophyll content. Semi-quantitative RT-PCR and Northern analysis showed that the transcript level of DHS was significantly lower in PVX-NbDHS infected plants. At the same time, the expression for eIF-5A, the target proteins of DHS in N. benthamiana, was concomitantly suppressed by semi-quantitative RT-PCR. and Western analysis. From the phenotypic feature of the infected plants and the reduced expression abundance of DHS and eIF-5A, we concluded that NbDHS plays important roles in plant growth, development and senescence. The possible application of DHS gene in genetic modification of crops and horticultural plants was discussed.
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