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Analysis of genetic variation in Ganoderma lucidum after space flight

文献类型: 外文期刊

作者: Qi, JJ 1 ; Ma, RC 2 ; Chen, XD 2 ; Lan, J 2 ;

作者机构: 1.Peking Union Med Coll, Chinese Acad Med Sci, Inst Med Plants, Beijing 100094, Peoples R China

2.Peking Union Med Coll, Chinese Acad Med Sci, Inst Med Plants, Beijing 100094, Peoples R China; Beijing Res Ctr Agrobiotechnol, Beijing 100089, Peoples R China

期刊名称:SPACE LIFE SCIENCES: BIODOSIMETRY, BIOMARKERS AND LATE STOCHASTIC EFFECTS OF SPACE RADIATION ( 影响因子:2.152; 五年影响因子:1.978 )

ISSN: 0273-1177

年卷期: 2003 年 31 卷 6 期

页码:

收录情况: SCI

摘要: A modified CTAB method was used in the extraction of total cellular DNA of Ganoderma lucidum. Four strains Cx, Ch, C3 and C4, and their counterparts, four space flown strains Sx, Xh, S3 and S4, were analysed by amplified fragment length polymorphism (AFLP) with several primer combinations. Polymorphic bands were detected between Sx and Cx, S3 and C3, respectively. Somatic incompatibility tests further confirmed their heterogeneity. However, no disparity between Sh and Ch, S4 and C4 was detectable. The results suggest that spaceflight may be used to accelerate breeding of Ganoderma lucidum strains for commercial cultivation. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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