文献类型： 外文期刊
作者： Dong, ZF 1 ; Ma, RC 1 ; Peng, YF 2 ; Guan, HS 3 ;
作者机构： 1.China Natl Ctr Biotechnol Dev, Beijing 100081, Peoples R China
2.Beijing Agrobiotechnol Res Ctr, Beijing 100089, Peoples R China
3.Chinese Acad Agr Sci, Inst Plant Protect, Beijing 100081, Peoples R China
关键词： biosafety;35S promoter;marker gene;vector backbone sequence;CRE/lox
期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）
ISSN： 0577-7496
年卷期： 2001 年 43 卷 7 期
页码：
收录情况： SCI
摘要： The biosafety of genetically engineered plants has been of concernment in society and science in recent years. The issue of 35S promoter of CaMV has been contentious because of its wide use in plant genetic engineering. The debate on the safety and potential risks of the 35S promoter will be discussed here. Some of concerns are expressed about the dissemination of antibiotic-resistance genes and vector backbone sequences. Various methods and strategies are currently being developed for the marker gene excision and elimination of vector backbone sequences from transgenic plants. In this review, the CRE/lox system which could get rid of the marker geens and vector backbone sequences will he discussed in detail. Advances in the research of the safety assessment of genetically modified plants using the CRE/lox system will also be described.
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