文献类型： 外文期刊

作者： Yang, W ¹ ; Zheng, YL ² ; Ni, S ² ; Wu, J ² ;

作者机构： 1.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China

2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China; Guizhou Acad Agr Sci, Inst Upland Food Crops, Guiyang 550006, Guizhou, Peoples R China; Guizhou Ctr Maize Engn Tech, Guiyang 550006, Guizhou, Peoples R China

关键词： opaque-2 alleles;replication slippage;SSR;unequal crossing over;Zea mays L;MOLECULAR ANALYSIS;ZEA-MAYS;LENGTH-POLYMORPHISMS;EUKARYOTIC GENOMES;REGULATORY LOCUS;MAJOR MECHANISM;MUTANT ALLELES;WILD-TYPE;DNA;OPAQUE-2

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recessive allelic variations were investigated at 3 microsatellite (SSR) sites within the O2 gene by using 14 inbred o2 lines and a wild-type line in maize. Among the 15 lines, allelic variations were observed at umc1066, phi057, and phi112 sites. Two alleles were found at the umc1066 site-a recessive allele with 2 perfect GCCAGA repeats and a dominant allele with 3 perfect repeats. Three alleles were found at the phi057 site-2 recessive alleles with 3 and 5 perfect GCC repeats, respectively, and another with 4 perfect repeats consistent with a dominant allele. At least 4 alleles exist at the phi 112 site-among which 1 recessive allele has a 1-bp deletion, another has a 15-bp deletion, and other has no PCR products compared to the dominant allele; all the alleles have unchanged AG repeats. The phi057 site in exon 6 was identified to be a hypervariable region in the coding sequence of the O2 gene, in addition to the 2 hypervariable regions in exon I previously reported. The primary mechanisms underlying the variations in repeat numbers and regions flanking the SSR within the O2 gene appear to be unequal crossing over and replication slippage. Furthermore, base substitution of SSR motif can create heteroalleles and modify the repeat number of SSR. The lysine content of kernel in the O2 and o2 lines correlates to a considerable extent with nucleotide variations at the umc1066, phi057, and phi112 sites. Our study suggests that it is best to use the 3 markers together in molecular marker-assisted selection for high-lysine maize materials.