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A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis

文献类型： 外文期刊

作者： Jang, In-Kwon 2 ; Meng, Xian-Hong 1 ; Seo, Hyung-Chul 2 ; Cho, Yeong-Rok 2 ; Kim, Bong-Rae 2 ; Ayyaru, Gopalakannan 2 ;

作者机构： 1.CAFS, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Natl Fisheries Res & Dev Inst, W Sea Mariculture Res Ctr, Taean 357945, Chungnam, South Korea

关键词： Breeding;Hatcheries;Infection;Polymerase Breeding;Hatcheries;Infection;Polymerase chain reaction;Stocking;copy number;white spot syndrome;Fenneropenaeus chinensis;White spot syndrome virus

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN：

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收录情况： SCI

摘要： In the present study, a highly sensitive and specific TaqMan real-time PCR was developed to quantify white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis. A total of159 individuals of wild F. chinensis brooders from 3 locations were captured and 210 postlarvae (PL sub(1) sub(-) sub(8)) were obtained from seven commercial hatcheries in 2007 in South Korea. The WSSV infections in 3 broodstocks showed a wide range, from 0 to 2.28x10 super(6) (with a mean of 1.50x10 super(4)) copies ng super(-) super(1) of DNA. Out of 159 brooders assayed, 39 (24.5%) were negative and 120 (75.5%) were positive; 153 (96.2%) showed less than 100 copies (mean 10.2 copies), 111 (69.8%) showed less than 10 copies and only 6 individuals (3.8%) showed high infections with a range of 2.36x10 super(2) to 2.28x10 super(6) copies ng super(-) super(1) of DNA. In 210 postlarvae, a range of 2.6 to 713.6 (with a mean of 220) copies g super(-) super(1) of DNA was observed. The mean WSSV copy number in the postlarvae was 7.9x10 super(5), which was equivalent to 8.5x10 super(5) copies mg super(-) super(1) of postlarvae weight. A total of 87.1% of postlarvae were WSSV positive and except for two hatcheries (H sub(4) and H sub(7)), the postlarvae of all the other five hatcheries were positive. Even postlarvae from the same hatchery, especially of hatcheries H sub(4) and H sub(5), showed a wide range of WSSV infection resulting in higher infections than other hatcheries. There were 34.3% of the postlarvae assayed in the present study that showed very low infection, with less than 10 copies ng super(-) super(1) of DNA. Based on our results, it is recommended to pre-screen broodstock or larvae for selective breeding, stocking in production systems or stock enhancement.

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