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Vegetative storage protein in Litchi chinensis, a subtropical evergreen fruit tree, possesses trypsin inhibitor activity

文献类型： 外文期刊

作者： Tian, Wei-Min 1 ; Peng, Shi-Qing 2 ; Wang, Xu-Chu 2 ; Shi, Min-Jing 2 ; Chen, Yue-Yi 3 ; Hu, Zheng-Hai;

作者机构： 1.Chinese Acad Sci, Rubber Res Inst, Key Lab Agr Minist Cultivat & Physiol Trop Crops, Danzhou 571737, Hainan, Peoples R China

2.Chinese Acad Sci, Rubber Res Inst, Key Lab Agr Minist Cultivat & Physiol Trop Crops, Danzhou 571737, Hainan, Peoples R China; NW Univ Xian, Inst Bot, Xian 710069, Peoples R China; Chinese Acad Trop Agr Sci, Natl Key Biotechnol Lab Trop Crops, Haikou 571101, Peoples R China

3.Chinese Acad Sci, Rubber Res Inst, Key Lab Agr Minist Cultivat & Physiol

关键词： defence;evergreen tree;Kunitz trypsin inhibitor;Litchi chinensis;nitrogen storage;vegetative storage protein.;METHYL JASMONATE;SOPHORA-JAPONICA;GENE-EXPRESSION;ACID-PHOSPHATASES;NITROGEN STORAGE;PARENCHYMA CELLS;SECONDARY PHLOEM;VACUOLE PROTEINS;SEASONAL-CHANGES;BARK

期刊名称：ANNALS OF BOTANY （ 影响因子：4.357； 五年影响因子：5.488 ）

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收录情况： SCI

摘要： Background and Aims Vegetative storage proteins (VSPs) are commonly bioactive in herbaceous plants but few VSPs with bioactivity have been identified in trees. In addition, information on the characterization of VSPs in evergreen trees is limited. The objective of this study was to characterize the VSPs with bioactivity in evergreen trees. Methods The VSP in lychee (Litchi chinensis), an evergreen fruit tree, was characterized by a combination of cytological, biochemical and molecular biological techniques. Key Results The VSP in lychee was a 22-kDa protein. It accumulated in the large central vacuoles of protein-storing cells (PSCs) in two distinguishable forms, granular and floccular. The PSCs were of a novel type. The 22-kDa protein is distributed in mature leaves, bark tissues of branches, trunk and large roots, paralleling the distribution of PSCs. Its homologues were present in mature seed. During young shoot development and fruiting, the 22-kDa protein decreased apparently, suggesting a nitrogen-storage function. The 22-kDa protein had several isoforms encoded by a small multigene family. One gene member, LcVSP1, was cloned. The LcVSP1 had no intron and contained a 675 bp open reading frame encoding a putative protein of 225 amino acids. LcVSP1 was homologous to Kunitz trypsin inhibitors. The 22-kDa protein inhibited trypsin and chymotrypsin, but had no inhibitory effect on subtilisin. Conclusions Lychee is rich in a 22-kDa VSP with trypsin inhibitor activity. The VSP plays an important role in nitrogen storage while its possible defensive function remains to be elucidated.

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