A primary sodium pump gene of the moderate halophile Halobacillus dabanensis exhibits secondary antiporter properties
文献类型： 外文期刊
作者： Yang, LF 1 ; Jiang, JQ 2 ; Zhang, B 2 ; Zhao, BS 2 ; Wang, L 3 ; Yang, SS;
作者机构： 1.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Minist Agr,Key Lab AgroMicrobial Resource & Appli, Beijing 100094, Peoples R China
2.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Minist Agr,Key Lab AgroMicrobial Resource & Appli, Beijing 100094, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Minist Agr, Key Lab Physiol Trop Crops, Hainan 571737, Peoples R China
3.China Agr Univ, Coll Biol Sci, Dept Microbiol & Immunol, Minist Agr,Key Lab AgroMicrobial Resource & Appli, Beij
关键词： moderate halophile;Halobacillus dabanensis;primary sodium pump;Na+/H+ antiporter;NADH-QUINONE OXIDOREDUCTASE;NA+-TRANSLOCATING NADH;ESCHERICHIA-COLI;COMPLEX-I;KLEBSIELLA-PNEUMONIAE;VIBRIO-ALGINOLYTICUS;MARINE BACTERIUM;NDH-I;TRANSPORTERS;CIRCULATION
期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）
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收录情况： SCI
摘要： The primary sodium pump has been proved to be involved in Na+ extrusion of bacteria. In our present study, a novel gene encoding a putative primary sodium pump was cloned from chromosomal DNA of moderate halophile Halobacillus dabanensis D-8 by functional complementation, which expression resulted in the growth of antiporter-deficient Escherichia coli strain KNabc in the presence of 0.2 M NaCl. The gene was sequenced and designated nap. The deduced amino acid sequence of Nap has 56% identity to NADH dehydrogenase of Bacillus cereus and 55% to NADH oxidase of Bacillus halodurans C-125. E coli KNabc carrying nap exhibited resistance to uncoupler CCCP (carbonyl-cyanide m-chlorophenylhydrazone). Everted membrane vesicles prepared from E. coli KNabc carrying nap exhibited secondary Na+/H+ antiporter activity, and nap also supported the growth of respiratory-deficient E coli ANN0222 lacking NADH dehydrogenase. Based on these results, we proposed that Nap possessed both characteristics of secondary Na+/H+ antiporter and primary sodium pump. (c) 2006 Elsevier Inc. All rights reserved.
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A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization
作者：Yang, LF;Jiang, JQ;Zhao, BS;Zhang, B;Feng, DQ;Lu, WD;Wang, L;Yang, SS
关键词：Halobacillus dabanensis;moderately halophilic bacterium;Na+/H+ antiporter;nhaH;ESCHERICHIA-COLI;VIBRIO-PARAHAEMOLYTICUS;BACILLUS-SUBTILIS;NHAB GENE;SP-NOV.;ALGINOLYTICUS;CIRCULATION;EXPRESSION;RESISTANCE;SEQUENCE
