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A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization

文献类型: 外文期刊

作者: Yang, LF 1 ; Jiang, JQ 2 ; Zhao, BS 2 ; Zhang, B 2 ; Feng, DQ 3 ; Lu, WD; Wang, L; Yang, SS;

作者机构: 1.China Agr Univ, Coll Biol Sci, Dept Microbiol, Beijing 100094, Peoples R China

2.China Agr Univ, Coll Biol Sci, Dept Microbiol, Beijing 100094, Peoples R China; Key Lab Agromicrobial Resource & Appl Minist Agr, Beijing, Peoples R China; Chinese Acad Trop Agr Sci, Rubber Res Inst, Hainan, Peoples R China; Key Lab Physiol Trop Crops Minist Agr, Hainan, Peoples R China

3.China Agr Univ, Coll Biol Sci, Dept Microbiol, Beijing 100094, Peoples R Chin

关键词: Halobacillus dabanensis;moderately halophilic bacterium;Na+/H+ antiporter;nhaH;ESCHERICHIA-COLI;VIBRIO-PARAHAEMOLYTICUS;BACILLUS-SUBTILIS;NHAB GENE;SP-NOV.;ALGINOLYTICUS;CIRCULATION;EXPRESSION;RESISTANCE;SEQUENCE

期刊名称:FEMS MICROBIOLOGY LETTERS ( 影响因子:2.742; 五年影响因子:2.856 )

ISSN:

年卷期:

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收录情况: SCI

摘要: A gene encoding a Na+/H+ antiporter was cloned from a chromosomal DNA of Halobacillus dabanensis strain D-8(T) by functional complementation. Its presence enabled the antiporter-deficient Escherichia coli strain KNabc to survive in the presence of 0.2 M NaCl or 5 mM LiCl. The gene was sequenced and designated as nhaH. The deduced amino-acid sequence of NhaH consists of 403 residues with a calculated molecular mass of 43 481 Da, which was 54% identical and 76% similar to the NhaG Na+/H+ antiporter of Bacillus subtilis. The hydropathy profile was characteristic of a membrane protein with 12 putative transmembrane domains. Everted membrane vesicles prepared from E. coli cells carrying nhaH exhibited Na+/H+ as well as Li+/H+ antiporter activity, which was pH-dependent with highest activities at pH 8.5-9.0 and at pH 8.5, respectively. Moreover, nhaH confers upon E. coli KNabc cells the ability to grow under alkaline conditions.

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