文献类型： 外文期刊

作者： Wang, P. ¹ ; Jiang, X. ¹ ; Jiang, Y. ¹ ; Hu, X. ¹ ; Hwang, H. ³ ;

作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao, Peoples R China

3.Jackson State Univ, Dept Biol, Jackson, MS USA

关键词： exo-polysaccharides;Isaria farinosa;mycelium;optimization;submerged fermentation;SUBMERGED CULTURE-CONDITIONS;PAECILOMYCES-TENUIPES C240;CORDYCEPS-MILITARIS;BIOPOLYMER PRODUCTION;SCIENTIFIC REDISCOVERY;CHEMICAL-PROPERTIES;SINENSIS;AERATION;FUNGI;EXOPOLYSACCHARIDES

期刊名称：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY （ 影响因子：2.162； 五年影响因子：1.901 ）

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收录情况： SCI

摘要： The optimal fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05 were investigated. The medium components and fermentation conditions were optimized according to the one at a time method, while the concentration of medium components was determined by the orthogonal matrix method. The results showed that the optimal fermentation medium was as follows: sucrose 3.5% (w/v), peptone 0.5%, yeast extract 0.2%, K2HPO4 0.1%, and MgSO4 0.05%. The suitable fermentation conditions were as follows: initial pH 7.0, temperature 25 degrees C, medium volume 75mL/250mL, inoculum volume 5% (v/v), time 5d. In such optimal nutrition and environmental conditions, the maximal mycelial yield was 2.124g/100mL after 4 day's fermentation, while maximal water-soluble exo-polysaccharides production reached 2.144g/L after 5 day's fermentation.