文献类型： 外文期刊

作者： Guo, HN ¹ ; Wu, JH ² ; Chen, XY ² ; Luo, XL ² ; Lu, R ² ; Shi, YJ ² ; Qin, HM ² ; Xiao, JL ² ; Tian, YC ² ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China

2.Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China; Shanxi Acad Agr Sci, Inst Cotton, Yuncheng 044000, Peoples R China

关键词： synthetic chimeric Cry1Ac gene;arrowhead proteinase inhibitor gene;insect-resistant transgenic cotton plants

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

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收录情况： SCI

摘要： A chimeric gene, Bt29K, composed of coding sequences of activated Cry1Ac insecticidal protein and an endoplasm reticulum-retarding signal peptide, was synthesized. A plant expression vector containing two expression cassettes for the Bt29K and API-B genes was constructed. These two insect-resistant genes were transferred into two cotton (Gossypiwn hirsutum L.) varieties (or lines) via Agrobacterium-mediated transformation and nine homozygous transgenic cotton lines showing a mortality of 90.0%-99.7% to cotton hairworm ( Heliothis armigera ) larvae and good agronomic traits were selected through six generations. Molecular biology analysis revealed that one or two copies of the insecticidal protein genes were integrated into the transgenic cotton genome and activated CrylAc and API-B protein expression was at a level of 0.17% and 0. 09% of the total soluble protein in the transgenic cotton leaves, respectively. Comparison of the insect-resistance of the homozygous lines expressing the activated chimeric Cry1Ac and API-B with that expressing Cry1Ac only revealed that the insect-resistance of the former is apparently higher than the latter. These results also indicate that the strategy to construct a plant expression vector expressing two different insect-resistant genes reported here is reasonable.