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Estimation of genetic variation in Cynodon dactylon accessions using the ISSR technique

文献类型： 外文期刊

作者： Huang, Chun-Qiong 1 ; Liu, Guo-Dao 1 ; Bai, Chang-jun 1 ; Wang, Wen-qiang 1 ; Zhou, Shao-yun 1 ; Yu, Dao-Qeng 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, KeyLab Trop Crops Germplasm Resources Utilizat, Minist Agr, Danzhou 571737, Peoples R China

关键词： primer;inter-simple sequence repeat marker;genetic variation;genetic relationship;fragment size;arithmetic average;accession data;genetic similarity coefficient;monomorphic band;scorable band

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

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收录情况： SCI

摘要： Genetic variation in 55 accessions of Cynodon dactylon was estimated using inter-simple sequence repeat (ISSR) markers. The plant materials used in this study originated from 17 countries. A total of 236 ISSR fragments were generated with 14 primers. Fragment sizes ranged from 200 to 3000 bp. All scorable bands were polymorphic in nature and none of the primers used produced monomorphic bands, indicating a high level of genetic variation in this grass. The accessions were found to be clustered into eight major groups through the unweighted pair-group method with arithmetic averages. Genetic similarity coefficients (GSC) among the 55 accessions ranged from 0.52 to 0.95. The results clearly indicate that a high level of variation exists in Cynodon accessions. This study shows that the ISSR technique is a reliable tool for differentiating Cynodon accessions and for determining the genetic relationships among them

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