Karyotype characterization of Epimedium species (Berberidaceae) by fluorescent banding and physical mapping of 45S and 5S rDNA
文献类型： 外文期刊
作者： Ying, Diao 2 ; Chen Qingfu 1 ; Lin Xianming 5 ; Liao Chaolin 5 ; Hu Zhongli 3 ; Wang, Youwei 2 ;
作者机构： 1.Guizhou Normal Univ, Sch Biol Technol & Engn, Inst Plant Genet & Breeding, Guiyang 550001, Peoples R China
2.Wuhan Univ, Coll Pharm, Wuhan 430072, Peoples R China
3.Wuhan Univ, Coll Life Sci, Minist Educ Plant Dev Biol, Key Lab, Wuhan 430072, Peoples R China
4.Chongqing Univ Arts & Sci, Coll Life Sci & Technol, Chongqing 402168, Peoples R China
5.Hubei Acad Agr Sci, Inst Chinese Herbal Med, Enshi 445000, Peoples R China
关键词： chromosome;Epimedium;FISH;PI-DAPI staining;karyotype;rDNA
期刊名称：CARYOLOGIA （ 影响因子：0.918； 五年影响因子：0.846 ）
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收录情况： SCI
摘要： Epimedium is a very important economic genus not only for medicinal usage but also for horticul-ture. We used combined PI-DAPI (CPD) staining techniques and fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes to investigate the karyotypic and cytogenetic chracteristics of Epimedium species. All the tested species had the same chromosome number 2n=2x=12 and the same karyotype asymmetry type 2A. Just one pair of satellite chromosomes were found in each Epimedium species. Two different karyotype formulae were identified in the tested eight species. The CPD banding patterns varied among the eight Epimedium spe-cies. In the double-target FISH analysis, the 45S rDNA probe revealed the loci closely to the secondary constric-tions of satellite chromosome pair and 5S rDNA were located on the short arms of one metacentric pairs in the specimens. The present results provide the basis for the molecular genetics in Epimedium.
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