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Identification and characterization of microsatellite markers from Musa balbisiana

文献类型： 外文期刊

作者： Wang, Jing-yi 2 ; Huang, Bing-zhi 4 ; Chen, Ye-yuan 5 ; Feng, Su-ping 1 ; Wu, Yao-ting 1 ;

作者机构： 1.Qiongzhou Univ, Sanya 572002, Hainan Province, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Trop Crop Biotechnol, Minist Agr, Haikou 571101, Hainan Province, Peoples R China

3.Hainan Univ, Coll Agr, Key Lab Trop Hort Plant Resources & Genet Improve, Minist Educ, Haikou 570228, Hainan Province, Peoples R China

4.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou 510640, Guangdong, Peoples R China

5.Chinese Acad Trop Agr Sci, Inst Trop Crops Genet Resources, Danzhou 571737, Hainan Province, Peoples R China

关键词： microsatellite marker;allele;point mutation;genetic diversity;cultivar identification;single-nucleotide polymorphism;length variation;loci transferability;cross-species-genera transferability

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Microsatellite sequences were determined from Musa balbisiana, a wild diploid species in China, using selectively amplified microsatellite analysis. Fourteen microsatellite loci were evaluated in a collection of 22 cultivated bananas and 11 wild species. A total of 77 alleles were detected with an average of 5.5 alleles and heterozygosity (H) = 0.355 per locus. Efficient loci transferability of M. balbisiana simple sequence repeat markers across other wild species of the genus was observed. Sequencing alleles at four loci showed that, apart from microsatellite length variation, point mutations and insertions/deletions were quite abundant, especially when comparing Musella lasiocarpa and Ensete glaucum with the other Musa species/subspecies. In the sequenced regions, some single-nucleotide polymorphisms were identified which distinguished M. lasiocarpa and E. glaucum from the other Musa species.

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