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Protein differential expression in the latex from Hevea brasiliensis between self-rooting juvenile clones and donor clones

文献类型: 外文期刊

作者: Li, Hui-Liang 1 ; Guo, Dong 1 ; Lan, Fang-Ying 1 ; Tian, Wei-Min 2 ; Peng, Shi-Qing 1 ;

作者机构: 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Minist Agr, Key Lab Trop Crop Biotechnol, Haikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Rubber, Key Lab Rubber Biol, Minist Agr, Danzhou 571737, Peoples R China

关键词: carbohydrate: metabolism;lipid: metabolism;protein: synthesis;expression;degradation;regulation;transport;molecular mechanism;energy metabolism;NCBI sequence data;signal translocation;latex;self-rooting juvenile clone;donor clone;nucleoside acid process

期刊名称:ACTA PHYSIOLOGIAE PLANTARUM ( 影响因子:2.354; 五年影响因子:2.711 )

ISSN:

年卷期:

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收录情况: SCI

摘要: To better understand molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones, a proteomic approach was used to profile protein changes in the latex between self-rooting juvenile clones and donor clones. Total soluble proteins were extracted from latex in self-rooting juvenile clones and donor clones. Two-dimensional gel electrophoresis (2-DE) was used to identify proteins that were differentially expressed in self-rooting juvenile clones and donor clones and image analysis was used to determine which proteins were up- or down-regulated. Twenty-four differentially expressed protein spots were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Among the 24 proteins identified, 13 proteins were up-regulated, 11 proteins were decreased in self-rooting juvenile clones. These proteins were classified as carbohydrate and energy metabolism, secondary metabolism, signal translocation, transcriptional regulation-related, protein synthesis and degradation, transport, nucleoside acid process, lipid metabolism. Perhaps, the present study contributes towards an understanding of the molecular mechanism underlying the difference between self-rooting juvenile clones and donor clones.

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