文献类型： 外文期刊
作者： Li, Zhengnan 1 ; Zhang, Lei 3 ; Che, Haiyan 4 ; Liu, Hongguang 3 ; Chi, Ming 3 ; Luo, Daquan 5 ; Li, Yan 1 ; Chen, Wang 1 ;
作者机构： 1.NW A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China
2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China
3.NW A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China
4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China
5.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737
关键词： 16S ribosomal DNA;Rsa I: 80449-06-5;Taq I;EcoR I;Hpa II: 81295-25-2;KpnI;16/23S intergenic spacer region;GenBank sequence data
期刊名称：PHYTOPARASITICA （ 影响因子：1.439； 五年影响因子：1.569 ）
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收录情况： SCI
摘要： A disease on parthenium weed (Parthenium hysterophorus L.) was observed in June 2008 in Danzhou of Hainan Province. Infected weeds showed phytoplasma-like associated symptoms such as severe stunting, excessive proliferation of shoots, inflorescence-clustering, green petal, small leaves and witches'-broom. The original cause of phytoplasma was further confirmed by polymerase chain reaction (PCR). PCR products of 1.8 kb were obtained using the universal primers pair (P1/P7) designed to amplify the entire 16S rDNA and the 16/23S intergenic spacer region in a direct PCR assay. The primers pair R16F2n/R2 was used to amplify a PCR product of 1.2 kb. Restriction fragment length polymorphism (RFLP) was used to analyze the partial 16S rDNA sequences (1.2 kb) of all phytoplasma DNA digested with five endonucleases (Kpn I, Hpa II, Taq I, Rsa I, EcoR I). The RFLP patterns of the strain were found to be identical with that of the reference peanut witches'-broom phytoplasma. Based on the RFLP data, it is suggested that the phytoplasma strain belongs to subgroup 16SrII-A. This is the first demonstration of a 16SrII-A group phytoplasma associated with parthenium weed.
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