您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A disease associated with phytoplasma in Parthenium hysterophorus

文献类型： 外文期刊

作者： Li, Zhengnan 1 ; Zhang, Lei 3 ; Che, Haiyan 4 ; Liu, Hongguang 3 ; Chi, Ming 3 ; Luo, Daquan 5 ; Li, Yan 1 ; Chen, Wang 1 ;

作者机构： 1.NW A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China

2.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China

3.NW A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737, Peoples R China

5.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Danzhou 571737

关键词： 16S ribosomal DNA;Rsa I: 80449-06-5;Taq I;EcoR I;Hpa II: 81295-25-2;KpnI;16/23S intergenic spacer region;GenBank sequence data

期刊名称：PHYTOPARASITICA （ 影响因子：1.439； 五年影响因子：1.569 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A disease on parthenium weed (Parthenium hysterophorus L.) was observed in June 2008 in Danzhou of Hainan Province. Infected weeds showed phytoplasma-like associated symptoms such as severe stunting, excessive proliferation of shoots, inflorescence-clustering, green petal, small leaves and witches'-broom. The original cause of phytoplasma was further confirmed by polymerase chain reaction (PCR). PCR products of 1.8 kb were obtained using the universal primers pair (P1/P7) designed to amplify the entire 16S rDNA and the 16/23S intergenic spacer region in a direct PCR assay. The primers pair R16F2n/R2 was used to amplify a PCR product of 1.2 kb. Restriction fragment length polymorphism (RFLP) was used to analyze the partial 16S rDNA sequences (1.2 kb) of all phytoplasma DNA digested with five endonucleases (Kpn I, Hpa II, Taq I, Rsa I, EcoR I). The RFLP patterns of the strain were found to be identical with that of the reference peanut witches'-broom phytoplasma. Based on the RFLP data, it is suggested that the phytoplasma strain belongs to subgroup 16SrII-A. This is the first demonstration of a 16SrII-A group phytoplasma associated with parthenium weed.

  • 相关文献

[1]Jishengella endophytica gen. nov., sp nov., a new member of the family Micromonosporaceae. Xie, Qing-Yi,Wang, Cheng,Wang, Rong,Qu, Zhi,Lin, Hai-Peng,Hong, Kui,Goodfellow, Michael.

[2]Ectopic over-expression of two apple Flowering Locus T homologues, MdFT1 and MdFT2, reduces juvenile phase in Arabidopsis. Li, W. M.,Tao, Y.,Yao, Y. X.,Hao, Y. J.,You, C. X.,Li, W. M.,Tao, Y.,Yao, Y. X.,Hao, Y. J.,You, C. X.,Li, W. M..

[3]Nonomuraea wenchangensis sp nov., isolated from mangrove rhizosphere soil. Wang, Fan,Xu, Xiao-Xiong,Qu, Zhi,Wang, Cheng,Lin, Hai-Peng,Xie, Qing-Yi,Ruan, Ji-Sheng,Hong, Kui,Wang, Fan,Sun, Ming.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台