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Optimization of polysaccharides extraction from Tetrastigma hemsleyanum Diels et Gilg using response surface methodology

文献类型： 外文期刊

作者： Shao, Qingsong 1 ; Deng, Yanming 2 ; Shen, Haijin 3 ; Fang, Hailing 4 ; Zhao, Xiaofang 1 ;

作者机构： 1.Zhejiang A&F Univ, Hangzhou 311300, Zhejiang, Peoples R China

2.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

3.Changzhou Hlth Coll, Changzhou 213000, Peoples R China

4.Jiangxi Acad Forestry, Nanchang 330032, Peoples R China

关键词： Extraction;Optimization;Polysaccharides;Response surface methodology;Tetrastigma hemsleyanum Diels et Gilg

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN：

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收录情况： SCI

摘要： A 15-run Box-Behnken design (BBD) was used to optimize the extraction conditions of polysaccharides from Tetrastigma hemsleyanum Diels et Gilg. Three factors such as extraction temperature (°C), extraction time (h), and ratio of water to raw material were investigated. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis and also examined using the appropriate statistical methods. The adjusted coefficient of determination (RAdj2) for the model was 0.9754, and the probability value (P= 0.001) demonstrated a high significance for the regression model. The optimum extraction conditions were found to be: optimized extraction temperature 83.3 °C, extraction time 1.55. h and ratio of water to raw material 29.48. Under these conditions, the mean extraction yield of polysaccharides was 5.182 ± 0.093%, which was in good agreement with the predicted model value.

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