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Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio

文献类型： 外文期刊

作者： Li, Yan 1 ; Xu, Peng 1 ; Zhao, Zixia 1 ; Wang, Jian 1 ; Zhang, Yan 1 ; Sun, Xiao-Wen 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： genes;Hind III: 81295-22-9;genome;GenBank sequence data;bacterial artificial chromosome library;sequencing success rate

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

ISSN：

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收录情况： SCI

摘要： A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.

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