Construction and Characterization of the BAC Library for Common Carp Cyprinus Carpio L. And Establishment of Microsynteny with Zebrafish Danio Rerio
文献类型: 外文期刊
作者: Li, Yan 1 ; Xu, Peng 1 ; Zhao, Zixia 1 ; Wang, Jian 1 ; Zhang, Yan 1 ; Sun, Xiao-Wen 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing 100141, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词: genes;Hind III: 81295-22-9;genome;GenBank sequence data;bacterial artificial chromosome library;sequencing success rate
期刊名称:MARINE BIOTECHNOLOGY ( 影响因子:3.619; 五年影响因子:3.322 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A bacterial artificial chromosome (BAC) library of common carp Cyprinus carpio L. was constructed as a part of ongoing common carp genome project, which is aiming assembly of common carp genome. The library, containing a total of 92,160 BAC clones with an average insert size of 141 kb, was constructed into the restriction site of Hind III on BAC vector CopyControl pCC1BAC, covering 7.7 X haploid genome equivalents. Three dimension pools and superpools of the BAC library were established and 23 positive clones of 14 targets were identified from one-fifth of the BAC library. Pilot project of BAC end sequencing was conducted on 2,688 BAC ends from 1,344 clones and harvested 2,522 high-quality Q20 sequences with average length of 677 bp. The sequencing success rate was 93.8% and pair-end success rate was 92.3%. A total of 212 microsyntenies had been established between common carp and zebrafish genomes as a trial for genome-wide comparative genomics in these two closely related species.
- 相关文献
作者其他论文 更多>>
-
Integration of transcriptome, histopathology, and physiological indicators reveals regulatory mechanisms of largemouth bass ( Micropterus salmoides) in response to carbonate alkalinity stress
作者:Hua, Jixiang;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Li, Yan;Dong, Yalun;Jiang, Bingjie;Xi, Bingwen;Qiang, Jun
关键词:Micropterus salmoides; Carbonate alkalinity stress; Tissue damage; Serum biological chemistry; RNA-seq
-
Quantitative evaluation of essential amino acids and omega-3 long-chain polyunsaturated fatty acids from global marine bivalve aquaculture
作者:Tan, Karsoon;Xu, Peng;Huang, Leiheng;Luo, Cong;Choong, Khaihang;Li, Zexin;Guo, Yu;Cheong, Kit-Leong
关键词:Bivalve aquaculture; Lipid; PUFA; EPA plus DHA; Protein
-
An idea for marine ranching planning based on ocean currents and its practice in Zhongjieshan Archipelago National Marine Ranching, China
作者:Tang, Shengming;Xu, Peng;Zhang, Shouyu;Gao, Junqi;Yin, Yuqi;Yin, Yuqi;Liang, Jun;Xu, Kaida;Jiang, Yazhou;Li, Jun;Feng, Jianlong
关键词:Maring ranching planning; Coastal currents; Zhongjieshan Archipelago; Zhoushan Archipelago; Drifters
-
River inflow and seawater intrusion shape distinct phytoplankton communities in jinghai bay, a coastal bay of the Yellow Sea
作者:Sui, Jinchang;Ding, Dongsheng;Zhang, Yan;Zhu, Lin;Cui, Zhengguo;Wei, Yuqiu;Sui, Jinchang;Ding, Dongsheng;Wei, Yuqiu;Sun, Jun
关键词:Phytoplankton; River inflow; Seawater intrusion; Jinghai bay
-
Multi-Omics Investigation of Fatty Acid Content Variations in Common Carp (Cyprinus carpio) Muscle: Integrating Genome, Transcriptome, and Lipid Profiling Data
作者:Wang, Kaikuo;Chen, Yingjie;Wang, Qi;Cao, Yiming;Zhao, Ran;Yu, Shuangting;Sun, Xiaoqing;Zhang, Yan;Li, Jiongtang
关键词:FA profiles; genome; transcriptome; fatty acids; common carp
-
Evidently diverse effects of silver nanoparticles on Vibrio parahaemolyticus across different estuarine water samples
作者:Yang, Qianqian;Hou, Xiangyi;Lu, Feng;Zhang, Yan;Zhang, Xuzhi;Yang, Qianqian;Zhang, Dahai;Chen, Zhixiang;Lin, Wentao;Schlensky, Nick
关键词:
-
Spatial Organization of Fish Assemblages and Its Relation to Environmental Factors in the Lower Yalong River, China
作者:Ru, Huijun;Wu, Xiangxiang;Xu, Bin;Zhang, Yan;Wu, Fan;Ma, Baoshan
关键词:fish assemblages; lower Yalong River; plateau species; spatial variations; stream order



