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Detection of Sequence-Specific Gene by Multi-Channel Electrochemical DNA Chips

文献类型： 外文期刊

作者： Zhang, Xuzhi 1 ; Ji, Xinming 2 ; Cui, Zhengguo 1 ; Yang, Bing 1 ; Huang, Jie 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Fudan Univ, State Key Lab ASIC & Syst, Dept Microelect, Shanghai 200433, Peoples R China

关键词： DNA chips;WSSV gene;Multi-channel electrochemical detection;Electroanalysis;Loop-mediated isothermal amplification

期刊名称：BULLETIN OF THE KOREAN CHEMICAL SOCIETY （ 影响因子：0.969； 五年影响因子：0.691 ）

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收录情况： SCI

摘要： Five-channel electrochemical chips were fabricated based on the Micro-electromechanical System (MEMS) technology and were used as platforms to develop DNA arrays. Different kinds of thiolated DNA strands, whose sequences were related to white spot syndrome virus (WSSV) gene, were separately immobilized onto different working electrodes to fabricate a combinatorial biosensor system. As a result, different kinds of target DNA could be analyzed on one chip via a simultaneous recognition process using potassium ferricyanide as an indicator. To perfonn quantitative target DNA detection, a limit of 70 nM (S/N=3) was found in the presence of 600 nM coexisting noncomplementary ssDNA. The real samples of loop-mediated isothermal amplification (LAMP) products were detected by the proposed method with satisfactory result, suggesting that the multichannel chips had the potential for a high effective microdevice to recognize specific gene sequence for point-of-care applications.

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