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Quantification of SPS mRNA Expression in Banana Fruit Ripened under Different Conditions Using Real-time RT-PCR

文献类型： 外文期刊

作者： Li, Wen 2 ; Li, Xue-ping 5 ; Shao, Yuan-zhi 4 ; Xie, Jiang-hui 4 ; Chen, Wei-xin 6 ; Jin, Zhi-qiang 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou 571101, Peoples R China

2.Educ Minist, Key Lab Protect & Dev Trop Plant Resources, Haikou 570228, Peoples R China

3.Hainan Univ, Coll Hort & Landscape, Haikou 570228, Peoples R China

4.Key Lab Postharvest Physiol & Freshkeeping Techno, Zhanjiang 524091, Peoples R China

5.S China Agr Univ, Coll Hort, Guangzhou 510642, Guangdong, Peoples R China

6.Guangdong Prov Key Lab Postharvest Sci & Technol, Guangzhou 510642, Guangdong, Peoples R China

7.Hainan Univ, Coll Food Sci, Haikou 570228, Peoples R China

8.Chinese Acad Trop Agr Sci, S Subtrop Crops Res Inst, Zhanjiang 524091, Peoples R China

关键词： banana fruit;SPS mRNA expression;real-time reverse transcriptase-PCR;quantification;ripening

期刊名称：FOOD SCIENCE AND BIOTECHNOLOGY （ 影响因子：2.391； 五年影响因子：2.108 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Real-time reverse transcriptase (RT)-PCR using SYBR GreenI was applied to quantify sucrose phosphate synthase (SPS) mRNA expression at different storage days during the natural ripening (with no treatment), inhibited ripening with ethylene absorbent and accelerated ripening by treatment with propylene of banana fruit. The results showed that expression level of SPS mRNA were significantly different between 3 kinds of ripening fruit. Expression of SPS mRNA in fruit treated with propylene was dramatically promoted, the peak of SPS mRNA expression in these fruits appeared 16 days earlier than that in fruit with natural ripening. On the contrary, when fruit applied with ethylene absorbent, SPS mRNA expression was remarkably inhibited, the amount of SPS mRNA expression were significantly lower than that in naturally ripened fruit. These results indicated that the expression level of SPS mRNA of banana fruit can be impacted by regulating ethylene evolution, and that close relationships may exist between SPS mRNA expression and SPS activity, fruit softening, respiration rate, and ethylene production during ripening of banana fruit.

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