Precursor Supply Increases the Accumulation of 4-Hydroxy-6-(4-hydroxyphenyl)-alpha-pyrone after NRPS-PKS Gene Expression
文献类型： 外文期刊
作者： Li, Wen 1 ; Fan, Jie 1 ; Liao, Ge 1 ; Yin, Wen-Bing 2 ; Li, Shu-Ming 1 ;
作者机构： 1.Philipps Univ Marburg, Inst Pharmazeut Biol & Biotechnol, Fachbereich Pharmazie, D-35037 Marburg, Germany
2.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China
3.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
期刊名称：JOURNAL OF NATURAL PRODUCTS （ 影响因子：4.05； 五年影响因子：4.603 ）
ISSN： 0163-3864
年卷期： 2021 年 84 卷 8 期
页码：
收录情况： SCI
摘要： Expression of a nonribosomal peptide synthetase-nonreducing polyketide synthase hybrid gene pcr10109 from Penicillium crustosum PRB-2 in Aspergillus nidulans led to the accumulation of 4-hydroxy-6-(4-hydroxyphenyl)-alpha-pyrone (1). Adding para-hydroxybenzoic acid into the medium in which the overexpressing mutant is growing increased the product yield up to 5-fold. This strategy could be helpful for heterologous gene expression experiments requiring special substrates for product formation.
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