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Genetic diversity of Cynodon radiatus assessed by sequence-related amplified polymorphism markers

文献类型： 外文期刊

作者： Huang, Chun-Qiong 1 ; Zhang, Yong-Fa 2 ; Liu, Guo-Dao 1 ; Bai, Chang-Jun 1 ; Wang, Wen-Qiang 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Key Lab Trop Crops Germplasm Resources Utilizat, Minist Agr, Danzhou 571737, Peoples R China

2.Energy Stn Rural Environm Protect Hainan Prov, Haikou 570203, Hainan, Peoples R China

关键词： Cynodon radiatus;Genetic diversity;SRAP markers;UPGMA cluster analysis;Principle coordinate analysis

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

ISSN：

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收录情况： SCI

摘要： Sequence-related amplified polymorphism (SRAP) markers were used to assess the genetic relatedness among 33 Cynodon radiatus accessions from different regions of China. Fifteen primer combinations were used to amplify specific C. radiatus genomic sequences. A total of 382 SRAP fragments were generated, with fragment sizes ranging from 200 to 1800 bp. Genetic similarity coefficients among the 33 accessions ranged from 0.53 to 0.95, with an average of 0.70. Cluster analysis by two methods, the unweighted pair-group method with arithmetic averages and the principle coordinate analysis, separated the accessions into 3 distinct groups. This study shows that the SRAP technique is a reliable tool for differentiation of C. radiatus accessions and for determination of the genetic relationships among them. (C) 2011 Elsevier Ltd. All rights reserved.

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