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Characterization of the LhcSR Gene Under Light and Temperature Stress in the Green Alga Ulva linza

文献类型: 外文期刊

作者: Dong, Meitao 1 ; Zhang, Xiaowen 2 ; Zhuang, Zhimeng 2 ; Zou, Jian 2 ; Ye, Naihao 2 ; Xu, Dong 2 ; Mou, Shanli 3 ; Liang, 1 ;

作者机构: 1.Qingdao Agr Univ, Qingdao 266109, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.SOA, Inst Oceanog 1, Key Lab Marine Bioact Subst, Qingdao 266061, Peoples R China

4.Qingdao Univ Sci & Technol, Qingdao 266042, Peoples R China

关键词: Ulva linza;LhcSR;Full-length;Expression analysis;Green tide

期刊名称:PLANT MOLECULAR BIOLOGY REPORTER ( 影响因子:1.595; 五年影响因子:2.042 )

ISSN:

年卷期:

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收录情况: SCI

摘要: As a green-tide-forming macroalga, Ulva linza is distributed worldwide and therefore subject to various environmental stresses. The LHCSR (also known as LI818 in green alga and LHCX in diatoms) protein is a stress-related member of the LHC family that plays an important role in photo-protective mechanism, which has been only found in algae. In this study, we cloned full-length cDNA sequence encoding the LhcSR gene from U. linza and analyzed its expression in response to different temperature and illumination gradients. The results showed that high light (HL) could enhance expression of LhcSR and that the expression level peaked at 3 h under HL. Similarly, the expression of LhcSR could also be induced by low temperature (LT). However, the expression patterns of LhcSR were quite different in response to LT and HL treatment. Specifically, the maximum gene expression under LT was much higher (11.8-fold) than under HL (5.4-fold) when compared to the expression under normal conditions. The upregulated expression of LhcSR lasted for 12 h under LT, but 3 h under HL. These data suggest that the LhcSR gene is involved in photoprotection in U. linza, and the results suggest a stronger link to LT. In addition, the discrepancy in expression under HL and LT was consistent with the ecological features of this alga, which only thrives during the cold season (featured as LT and low light).

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