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Reverse transcription loop-mediated isothermal amplification of RNA for sensitive and rapid detection of southern rice black-streaked dwarf virus

文献类型： 外文期刊

作者： Zhou, Tong 1 ; Du, Linlin 1 ; Fan, Yongjian 1 ; Zhou, Yijun 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Plant Protect, Diag & Detect Ctr Plant Virus Dis, Nanjing 210014, Jiangsu, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China

关键词： SRBSDV;RT-LAMP;Detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

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收录情况： SCI

摘要： Southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in Southeast Asia. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of SRBSDV using total RNA extracted from rice tissues and the insect pest, white-backed planthopper. The assay was based on a set of four primers matching a total of six sequences in the 59 region of SRBSDV genome. Presence of the virus could be detected in RT-LAMP reactions containing 1.2 x 10(-6) mu g of a total RNA extract, which was ten times more sensitive than a classical RT-PCR assay. The SRBSDV could be distinguished from the closely related rice black-streaked dwarf virus (RBSDV) by this method, indicating a high degree of specificity. This simple and sensitive RT-LAMP assay shows potential for detection of SRBSDV in field samples of hosts or vectors at a relatively low cost. (C) 2011 Elsevier B.V. All rights reserved.

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