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Isolation and characterization of gene-derived single nucleotide polymorphism (SNP) markers in Scylla paramamosain

文献类型： 外文期刊

作者： Ma, Hongyu 1 ; Ma, Qunqun 1 ; Ma, Chunyan 1 ; Ma, Lingbo 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Marine & Estuarine Fisheries Resources &, Minist Agr, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

关键词： Mud crab;Scylla paramamosain;Single nucleotide polymorphism;SNaPshot assay

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

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收录情况： SCI

摘要： Single nucleotide polymorphisms (SNPs) are thought to be well suitable for genetic and evolutionary studies. In this study, we reported the first set of SNP markers in a commercially important crab species, Scylla paramamosain. A total of 12,500 basepairs high quality DNA sequences were obtained from 15 genes, and thirty-seven SNPs were identified, representing one SNP every 338 base pairs. Twenty-four SNPs were successfully genotyped in a single population. All loci had two alleles and the minor allele frequency ranged from 0.02 to 0.44. The observed and expected heterozygosity ranged from 0.04 to 0.59 and from 0.04 to 0.50, respectively. No significant departures from Hardy–Weinberg equilibrium at each locus was found. The linkage disequilibriumwas detected in six loci pairs, but absent after sequential Bonferroni correction. These SNP markers will provide a useful addition to the genetic tools for genetic and evolutionary studies for S. paramamosain.

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[1]Identification of a trypsin gene from Scylla paramamosain and its expression profiling during larval development. Jiang, Keji,Zhang, Fengying,Zhang, Dan,Zhang, Yong,Qiao, Zhenguo,Ma, Lingbo,Tao, Qichang,Pi, Yan. 2011

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[19]Late Pleistocene population expansion of Scylla paramamosain along the coast of China: A population dynamic response to the Last Interglacial sea level highstand. He, Lijun,Jiang, Chaojun,Qiao, Zhenguo,Zhang, Aibing,Weese, David,Zhu, Chaodong.

[20]Comparative Transcriptome Analysis Provides Insights into Differentially Expressed Genes and Long Non-Coding RNAs between Ovary and Testis of the Mud Crab (Scylla paramamosain). Yang, Xiaolong,Lin, Fan,Wu, Qingyang,Zhang, Yueling,You, Cuihong,Liu, Wenhua,Cheng, Yinwei,Shi, Xi,Wang, Shuqi,Ma, Hongyu,Ikhwanuddin, Mhd,Li, Xincang. 2018

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