文献类型: 外文期刊
作者: Huang, Jing 1 ; Ma, Guojian 1 ; Zhu, Mengjin 1 ; Pan, Jianzhi 4 ; Zhang, Wenchang; Zhao, Shu-Hong 1 ;
作者机构: 1.Huazhong Agr Univ, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Hubei, Peoples R China
2.Huazhong Agr Univ, Key Lab Swine Genet & Breeding, Minist Agr, Wuhan 430070, Hubei, Peoples R China
3.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet, Hangzhou 310021, Zhejiang, Peoples R China
4.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet, Hangzhou 310021, Zhejiang, Peoples R C
关键词: Pig;STAT4;STAT6;Genomic structure;Localization;SNP
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Signal transducers and activators of transcription (STATs) are members of a recently identified family of transcription factors that activate gene transcription in response to a number of different cytokines. STAT4 and STAT6 were activated by interleukin (IL)-12 and IL-4 stimulation, which were important for the generation of Th1 and Th2 cells. In this study, we cloned the cDNA sequences and analyzed the genomic structure of porcine STAT4 (poSTAT4) and STAT6 (poSTAT6) genes. Chromosome localization assigned these two genes to SSC15 and SSC5, and they were most closely linked to maker SWR1002 and DK. The RT-PCR revealed that both genes were expressed in eight diverse tissues, with the highest level in small intestine, followed by lung, kidney, muscle and stomach, whereas expressions in heart, liver and spleen were relatively weak. Transient transfection indicated that poSTAT4 and poSTAT6 proteins distributed throughout the whole porcine hip artery endothelial cell. A single nucleotide polymorphism (A/G), which can be recognized by restriction enzyme TaiI, was identified at the 3' untranslated region of poSTAT6, and genotyping results showed apparent variation in allele frequency between Chinese indigenous and western breeds.
- 相关文献
作者其他论文 更多>>
-
CRISPR/Cas9-meditated gene knockout in pigs proves that LGALS12 deficiency suppresses the proliferation and differentiation of porcine adipocytes
作者:Wu, Wenjing;Yin, Yajun;Zhang, Jin;Huang, Jing;Pan, Jianzhi;Yang, Ruifei;Li, Qiuyan;Zhang, Jin
关键词:LGALS12; CRISPR/Cas9; Knockout; Pig fat
-
The Influence of Increasing Roughage Content in the Diet on the Growth Performance and Intestinal Flora of Jinwu and Duroc x Landrace x Yorkshire Pigs
作者:Xu, Gaili;Chen, Wenduo;Zhao, Ayong;Xu, Gaili;Chen, Wenduo;Zhao, Ayong;Huang, Jing;Pan, Jianzhi;Yu, Fuxian
关键词:roughage diet; Jinwu pig; Duroc x Landrace x Yorkshire pig; roughage tolerance; gut microbiota
-
Characterization of Zizania latifolia polysaccharide-corn starch composite films and their application in the postharvest preservation of strawberries
作者:Huang, Jing;Wang, Yuhua;Wu, Weijie;Niu, Ben;Fang, Xiangjun;Chen, Hangjun;Gao, Haiyan
关键词:Polysaccharide extraction; Antioxidant film; Storage; Bio-based polymers; Shelf-life extension
-
Sulfur dioxide maintains storage quality of table grape (Vitis vinifera cv 'Kyoho') by altering cuticular wax composition after simulated transportation
作者:Li, Zhenbiao;Huang, Jing;Yang, Mingyi;Li, Dong;Xu, Yanqun;Li, Li;Luo, Zisheng;Li, Zhenbiao;Huang, Jing;Yang, Mingyi;Li, Dong;Li, Li;Luo, Zisheng;Chen, Hangjun;Xu, Yanqun;Li, Li;Luo, Zisheng;Chen, Jianye;Wu, Bin;Wu, Bin
关键词:Cuticular wax; Table grape; Simulated transportation; Quality; SO2
-
Protective efficacy of Toxoplasma gondii GRA12 or GRA7 recombinant proteins encapsulated in PLGA nanoparticles against acute Toxoplasma gondii infection in mice
作者:Sun, Hong-chao;Fu, Yuan;Shi, Tuan-yuan;Deng, Pu-ming;Deng, Jin-hua;Qi, Meng;Xie, Rong-hui;Huang, Jing
关键词:GRA7; GRA12; nanoparticles; PLGA; Toxoplasma gondii; vaccine
-
Pathogenicity and drug resistance of the Eimeria tenella isolate from Yiwu, Zhejiang province, eastern China
作者:Sun, Hongchao;Su, Xinyao;Fu, Yuan;Shi, Tuanyuan;Su, Xinyao;Hao, Lili;Zhou, Wei;Zhou, Zhijin;Huang, Jing;Wang, Yimin
关键词:Key words; Eimeria tenella; isolate; pathogenicity; drug resistance
-
Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage
作者:Huang, Jing;Wu, Weijie;Fang, Xiangjun;Han, Yanchao;Niu, Ben;Gao, Haiyan;Fang, Xiangjun;Chen, Hangjun;Han, Yanchao
关键词:Zizania latifolia; cell wall polysaccharide; cell wall metabolic enzymes; Pearson's correlation