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Expression, characterization and 2,4,6-trichlorophenol degradation of laccase from Monilinia fructigena

文献类型: 外文期刊

作者: Bao, Wenhua 1 ; Peng, Rihe 1 ; Zhang, Zhen 2 ; Tian, Yongsheng 1 ; Zhao, Wei 1 ; Xue, Yong 1 ; Gao, Jianjie 1 ; Yao, Quan 1 ;

作者机构: 1.Shanghai Acad Agr Sci, Agrobiotechnol Res Ctr, Shanghai 201106, Peoples R China

2.Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

关键词: Laccase;Pichia pastoris;Monilinia fructigena;Trichlorophenol

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A novel laccase gene from Monilinia fructigena was synthesized chemically according to the yeast bias codon and integrated into the genome of Pichia pastoris GS115 by electroporation. The expressed enzyme was recovered from the culture supernatant and purified. The result of enzyme activity assay and SDS-PAGE demonstrated that the recombinant laccase was induced and extracellularly expressed in P. pastoris. Main biochemical properties of this laccase, such as thermodependence and thermostability, optimal pH and pH stability, and the effect of metal ions and inhibitors, were characterized. With 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate (ABTS) as the substrate, MfLcc had its optimal pH at 3.5 and optimal temperature at 45A degrees C. The Km values of the ABTS, guaiacol were 0.012 and 0.016 Mm, respectively, and the corresponding V (max) values are 243.9 and 10.55 Um min(-1) mg(-1), respectively. The recombinant laccase degraded 80% 2,4,6-trichlorophenol after 8 h under the optimal conditions. The recombinant strain and its laccase can be considered as candidate for treating waste water polluted with trichlorophenols.

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