Molecular analysis of a ras-like nuclear (Ran) gene from Penaeus monodon and its expression at the different ovarian stages of development
文献类型: 外文期刊
作者: Zhou, FaLin 1 ; Zheng, Liming 1 ; Yang, QiBin 1 ; Qiu, Lihua 1 ; Huang, JianHua 1 ; Su, Tiannfeng 1 ; Jiang, ShiGui 1 ;
作者机构: 1.CAFS, S China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.S China Agr Univ, Inst Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
关键词: Molecular cloning;Ran;mRNA expression;Penaeus monodon
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
ISSN:
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收录情况: SCI
摘要: In the present study, a ras-like nuclear (Ran) gene was obtained from the ovary and neurosecretory organ in eyestalk cDNA library of black tiger prawn (Penaeus monodon). The full-length black tiger prawn Ran (PmRan) cDNA consisted of 1140 nucleotides including an open reading frame (ORF) 648 bp, a 5' untranslated region (5'UTR) of 117 bp and a 3'UTR of 375 bp with a polyadenylation signal sequence "aataaa" and a poly (A) tail. The ORF encoded a peptide of 215 amino acids with molecular mass 24.6 kDa and a theoretical isoelectric point of 7.39. ScanProsite analysis indicated that PmRan protein sequence contained a small GTPase Ran family motif. Homology analysis of the deduced amino acid sequence of the PmRan with other known Ran sequences by MatGAT software revealed that the PmRan show very high homology with the sequences of other animals (92.1-98.6% similarity, 85.6-98.1% identity). Analysis of the tissue expression pattern of the PmRan gene showed that the PmRan mRNA was expressed in all tested tissues, including hepatopancreas, ovary, muscle, intestine, neurosecretory organ in eyestalk, neurosecretory organ in brain, stomach, and heart, with the highest levels in ovary. Furthermore, the PmRan expression was found to be high level in the six ovarian stages of development. The results indicated PmRan might play an important role in ovarian development.
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