Molecular characterization of a novel 14-3-3 protein gene (Hb14-3-3c) from Hevea brasiliensis
文献类型: 外文期刊
作者: Yang, Zi-Ping 1 ; Li, Hui-Liang 1 ; Guo, Dong 1 ; Tian, Wei-Min 2 ; Peng, Shi-Qing 1 ;
作者机构: 1.Chinese Acad Trop Agr Sci, Key Lab Trop Crop Biotechnol, Minist Agr, Inst Trop Biosci & Biotechnol, Haikou 571101, Peoples R China
2.Chinese Acad Trop Agr Sci, Key Lab Rubber Biol, Minist Agr, Inst Rubber, Danzhou 571737, Peoples R China
关键词: 14-3-3 protein;Hevea brasiliensis;Jasmonate;Heavy metal ions;Interaction partner
期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )
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收录情况: SCI
摘要: The cDNA encoding a 14-3-3 protein, designated as Hb14-3-3c, was isolated from Hevea brasiliensis. Hb14-3-3c was 1,269 bp long containing a 795 bp open reading frame encoding a putative protein of 264 amino acids, flanked by a 146 bp 5'UTR and a 328 bp 3' UTR. The predicted molecular mass of Hb14-3-3c is 29.67 kDa, with an isoelectric point of 4.52 and the deduced protein showed high similarity to the 14-3-3 protein from other plant species. Expression analysis revealed more significant accumulation of Hb14-3-3c transcripts in latex than in leaves, buds and flowers. The transcription of Hb14-3-3c in latex was induced by jasmonate and ethephon. Overproduction of recombinant Hb14-3-3c protein gave the Escherichia coli cells more tolerance on Co2+, Cu2+ and Zn2+. Through yeast two-hybrid screening, 11 interaction partners of the Hb14-3-3c, which are involved in rubber biosynthesis, stress-related responses, defence etc., were identified in rubber tree latex. Taking these data together, it is proposed that the Hb14-3-3c may participate in regulation of rubber biosynthesis. Thus, the results of this study provide novel insights into the 14-3-3 signaling related to rubber biosynthesis, stress-related responses in rubber tree.
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