Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms
文献类型： 外文期刊
作者： Jiang, Liang 1 ; Wang, Genhong 1 ; Cheng, Tingcai 1 ; Yang, Qiong 2 ; Jin, Shengkai 1 ; Lu, Gai 1 ; Wu, Fuquan 2 ; Xiao, 1 ;
作者机构： 1.Southwest Univ, State Key Lab Silkworm Genome Biol, Chongqing 400715, Peoples R China
2.Guangdong Acad Agr Sci, Sericulture & Farm Prod Proc Res Inst, Guangzhou 510610, Guangdong, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
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收录情况： SCI
摘要： Transgenic technology is a powerful tool for improving disease-resistant species. Bmlipase-1, purified from the midgut juice of Bombyx mori, showed strong antiviral activity against B. mori nucleopolyhedrovirus (BmNPV). In an attempt to create an antiviral silkworm strain for sericulture, a transgenic vector overexpressing the Bmlipase-1 gene was constructed under the control of a baculoviral immediate early-1 (IE1) promoter. Transgenic lines were generated via embryo microinjection. The mRNA level of Bmlipase-1 in the midguts of the transgenic line was 27.3 % higher than that of the non-transgenic line. After feeding the silkworm with different amounts of BmNPV, the mortality of the transgenic line decreased to approximately 33% compared with the non-transgenic line when the virus dose was 10 6 OB/larva. These results imply that overexpressing endogenous antiviral genes can enhance the antiviral resistance of silkworms.
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