Lowering the Blood Glucose of Diabetes Mellitus Mice by Oral Administration with Transgenic Human Insulin-like Growth Factor I Silkworms
文献类型： 外文期刊
作者： Xue, Renyu 1 ; Wang, Yang 1 ; Cao, Guangli 1 ; Pan, Zhonghua 1 ; Zheng, Xiaojian 1 ; Zhou, Wenlin 3 ; Gong, Chengliang 1 ;
作者机构： 1.Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Peoples R China
2.Soochow Univ, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China
3.Zhejiang Acad Agr Sci, Sericulture Res Inst, Hangzhou 310021, Zhejiang, Peoples R China
关键词： transgenic silkworm;hlGF-I;DM mice;reducing blood glucose
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
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收录情况： SCI
摘要： To evaluate the biological activity of the posterior silk glands of transgenic silkworms expressing human insulinlike growth factor I (hIGF-l), we bred hIGF-I-transgenic silkworms through eight generations by continuously selecting with green fluorescence and G418. The G8 transgenic silkworms were confirmed by polymerase chain reaction and dot blotting, and their posterior silk glands were removed from the fifth instar larvae to make freeze-dried powders. Enzyme-linked immunosorbent assay results showed that the expression level of hIGF-I in the posterior silk glands of G8 transgenic silkworm is approximately 493 ng/g of freeze-dried powder. When the freeze-dried powder was administrated by gavage to diabetes mellitus (DM) mice, the blood glucose in DM mice significantly decreased (P < 0.05) in a time- and dose-dependent manner compared with that of DM mice orally administrated with distilled water and normal freeze-dried powders made of untreated silk glands. These results demonstrated that hIGF-I expressed in posterior silk glands of transgenic silkworms could reduce blood glucose by oral administration.
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