Isolation and characteristics of polymorphic microsatellite markers in Pennahia argentatus
文献类型: 外文期刊
作者: Ma, Chunyan 1 ; Ma, Hongyu 1 ; Xu, Zhen 1 ; Ma, Lingbo 1 ; Shen, Anglv 1 ; Feng, Nana 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Key Lab E China Sea & Ocean Fishery Resources Exp, Minist Agr, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
3.Shanghai Oce
关键词: Pennahia argentatus;Microsatellite markers;Polymorphism;5′ anchored PCR
期刊名称:BIOCHEMICAL SYSTEMATICS AND ECOLOGY ( 影响因子:1.381; 五年影响因子:1.351 )
ISSN:
年卷期:
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收录情况: SCI
摘要: In this study, we isolated 12 novel polymorphic microsatellite markers in Pennahia argentatus by using the 5' anchored PCR technique. The characteristics of these loci were estimated by using a sample of 30 individuals. A total of 69 alleles were detected with an average of 5.75 alleles per locus. The PIC value for the 12 microsatellite loci ranged from 0.1202 to 0.8466. The observed and expected heterozygosity per locus ranged from 0.1379 to 0.9333 and from 0.1307 to 0.8784, with an average of 0.5997 and 0.6083, respectively. One loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.0042) and no significant linkage disequilibrium between pairs of loci was found. This study will be useful for the analysis of population genetic diversity, and the management of this important fish resource. (C) 2012 Elsevier Ltd. All rights reserved.
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