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Identification of a pou2 ortholog in Chinese sturgeon, Acipenser sinensis and its expression patterns in tissues, immature individuals and during embryogenesis

文献类型： 外文期刊

作者： Ye, Huan 1 ; Du, Hao 1 ; Chen, Xi-Hua 1 ; Cao, Hong; Liu, Tao 1 ; Li, Chuang-Ju 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China

4.Chinese Acad Fish

关键词： Chinese sturgeon;Aspou2;Cloning;Gene expression;RT-PCR

期刊名称：FISH PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：2.794； 五年影响因子：2.876 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The class V POU family genes, including pou5f1 and pou2, encode transcription factors critical for the maintenance of pluripotency in embryonic stem cells (ESC) and germ line cells in vertebrates. In the present study, the full-length cDNA of a pou2 ortholog in A. sinensis, Aspou2, was cloned and sequenced. This cDNA sequence is 2,853 base pairs in length and encodes a peptide of 431 amino acid residues. A comparison of the deduced amino acid sequence of Aspou2 with that of other vertebrate species showed that they were highly conserved in the POU domain, which shared 88 and 90% identity with that of zebrafish and medaka, respectively, and was 69, 67 and 67% identical to frog, mouse and human, respectively. RT-PCR analysis revealed that Aspou2 was detected in all tissues examined except for the liver, and high mRNA levels of Aspou2 were found in the muscle, pituitary and brain. During the embryogenesis and early larval development, the expression level of Aspou2 mRNAs decreased gradually apart from 1-day larvae that were not observed. Furthermore, Aspou2 seemed to raise with the development of gonads of immature Chinese sturgeons. These results suggested the possible involvement of Aspou2 in the nonpluripotent cells, pluripotent cells, embryogenesis, and gonad development.

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