文献类型： 外文期刊
作者： Guo, Xinwei 1 ; Zhang, Xiuhai 1 ; Wu, Zhongyi 1 ; Luo, Chang 1 ; Cheng, Xi 1 ; Huang, Conglin 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Chrysanthemum lavandulifolium;High molecular weight DNA;Phenolic compound
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： We developed an improved method for preparing high molecular weight DNA (HMW-DNA) from leaves of Chrysanthemum lavandulifolium that has high content of phenolic compounds. Compared with three previous methods for preparing HMW-DNA, only with the improved method a considerable yield of HMW-DNA more than 2 Mb was achieved. The improved method utilizes a modified buffer system including 1% (v/v) beta-mercaptoethanol, 4 mM spermidine and 6% (w/v) polyvinylpyrrolidone (PVP)-40 and a centrifugal speed of 1600 x g to extract and suspend HMW-DNA. With the improved method, phenolic compounds were successfully removed and antioxidants protected HMW-DNA from oxidation and degradation. No difference on the yield of DNA was observed with different lysis times. The HMW-DNA can be partially digested to 23-80 kb in size, and is suitable for artificial chromosome library construction
- 相关文献
作者其他论文 更多>>
-
Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker
作者：Jin, Ge;Zhang, Xiuhai;Yu, Shiyin;Du, Yunpeng;Zhang, Mingfang;Jin, Ge;Zhao, Chunli;Yu, Shiyin;Wang, Meixian
关键词：
-
Functional identification and characterization of two flavonoid glycosyltransferases ZmUGT84A3 and ZmUGT84A4 from maize
作者：Ke, Zhao;Lu, Min;Ke, Zhao;Zheng, Dengyu;She, Meng;Zhang, Shipeng;Wu, Zhongyi;Zhang, Zhongbao;She, Meng
关键词：Uridine diphosphate glycosyltransferases; Maize; Flavonoid; Luteolin; In vitro experiments
-
Auxin regulates bulbil initiation by mediating sucrose metabolism in Lilium lancifolium
作者：Xin, Yin;Chen, Xi;Liang, Jiahui;Wu, Jingxiang;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Xin, Yin;Wang, Shaokun;Pan, Wenqiang;Wu, Jingxiang;Wu, Jian;Chen, Xi;Yu, Xiaonan;Zaccai, Michele
关键词：
-
Exogenous application of salicylic acid improves eradication of apple stem grooving virus and apple chlorotic leaf spot virus in apple
作者：Ma, Xiao-Yan;Li, Min-Ji;Ma, Xiao-Yan;Li, Jin-Wei;Li, Qing;Yan, Zi-Han;Cheng, Xi;Bao, Lu;Zhang, Dong;Wang, Min-Rui;Hamborg, Zhibo
关键词：Viral diseases; Thermotherapy; Cryotherapy; Shoot tip culture
-
Cloning, Expression, and Functional Characterization of Two Highly Efficient Flavonoid-di-O-glycosyltransferases ZmUGT84A1 and ZmUGT84A2 from Maize (Zea mays L.)
作者：Sun, Xiaorong;Ke, Zhao;Zheng, Dengyu;She, Meng;Wu, Zhongyi;Zhang, Zhongbao;Ke, Zhao;She, Meng;Li, Qing X.
关键词：flavonoid-di-O-glycosyltransferase; ZmUGT84A1; ZmUGT84A2; glycoxidation; glycoside; maize
-
Transcriptomic and metabolomic analyses reveal that MYB transcription factors regulate anthocyanin synthesis and accumulation in the disc florets of the anemone form of chrysanthemum morifolium
作者：Liu, Hua;Chen, Haixia;Lu, Jie;Chen, Dongliang;Luo, Chang;Cheng, Xi;Huang, Conglin;Chen, Haixia;Chen, Xiaoxi;Lu, Jie
关键词：Chrysanthemum morifolium; Disc florets; Anthocyanin biosynthesis; Whole-transcriptome analysis; Differentially expressed genes; Metabolomic analysis; Anemone-form chrysanthemum
-
A Modified Method for Transient Transformation via Pollen Magnetofection in Lilium Germplasm
作者：Zhang, Mingfang;Xue, Jing;Du, Yunpeng;Chen, Xuqing;Yang, Fengping;Zhang, Xiuhai;Ma, Xu;Jin, Ge;Han, Dongyang;Zhao, Chunli
关键词：Lilium regale; genetic transformation system; pollen magnetofection; PBI121 and pYBA1132 transformation
