文献类型： 外文期刊
作者： Guo, Xinwei 1 ; Zhang, Xiuhai 1 ; Wu, Zhongyi 1 ; Luo, Chang 1 ; Cheng, Xi 1 ; Huang, Conglin 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing 100097, Peoples R China
关键词： Chrysanthemum lavandulifolium;High molecular weight DNA;Phenolic compound
期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： We developed an improved method for preparing high molecular weight DNA (HMW-DNA) from leaves of Chrysanthemum lavandulifolium that has high content of phenolic compounds. Compared with three previous methods for preparing HMW-DNA, only with the improved method a considerable yield of HMW-DNA more than 2 Mb was achieved. The improved method utilizes a modified buffer system including 1% (v/v) beta-mercaptoethanol, 4 mM spermidine and 6% (w/v) polyvinylpyrrolidone (PVP)-40 and a centrifugal speed of 1600 x g to extract and suspend HMW-DNA. With the improved method, phenolic compounds were successfully removed and antioxidants protected HMW-DNA from oxidation and degradation. No difference on the yield of DNA was observed with different lysis times. The HMW-DNA can be partially digested to 23-80 kb in size, and is suitable for artificial chromosome library construction
- 相关文献
作者其他论文 更多>>
-
Auxin regulates bulbil development by affecting carbohydrate metabolism in Lilium lancifolium
作者：Wang, Mengdi;Li, Jiamin;Zhang, Xuemin;Kang, Shengnan;Yu, Xiaonan;Wang, Mengdi;Li, Jiamin;Pan, Wenqiang;Sang, Qianzi;Liang, Jiahui;Zhang, Xuemin;Kang, Shengnan;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Wang, Mengdi;Li, Jiamin;Pan, Wenqiang;Sang, Qianzi;Liang, Jiahui;Zhang, Xuemin;Kang, Shengnan;Zhang, Mingfang;Zhang, Xiuhai;Du, Yunpeng;Sang, Qianzi
关键词：Lilium lancifolium; Bulbil development; Transcriptome; Auxin; Carbohydrate metabolism
-
Salicylic acid influences the resistance of colored calla lily to soft rot (Pectobacterium carotovorum) via the TGA2-LNC88940-miR528-SOD module
作者：Wang, Yi;Wang, Di;Gou, Rongxin;Jiang, Yin;Zeng, Zhen;Zhang, Xiuhai;Wei, Zunzheng;Yang, Tuo;Wang, Di;Gou, Rongxin;Jiang, Yin;Zhang, Guojun
关键词：soft rot;
Zantedeschia elliottiana ; lncRNA; miRNA; salicylic acid -
The giant genome of lily provides insights into the hybridization of cultivated lilies
作者：Liang, Yuwei;Gao, Qiang;Zhang, Liangsheng;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Li, Fan;Duan, Qing;Li, Shenchong;Jin, Chunlian;Zhang, Peihua;Wang, Jihua;Du, Yunpeng;Pan, Wenqiang;Zhang, Xiuhai;Zhang, Mingfang;Wu, Jian;Pan, Wenqiang;Wang, Shaokun;Song, Xiaoming;Zhong, Linlin;Zhang, Fan;Li, Yan;Wang, Zhiwei;Li, Danqing;Gu, Yang;Chen, Zhong-Hua;Chen, Zhong-Hua;Mayer, Klaus F. X.;Mayer, Klaus F. X.;Zhou, Xiaofan;Zhang, Liangsheng;Zhang, Liangsheng
关键词：
-
The TIFY transcription factor ZmJAZ13 enhances plant tolerance to drought and salt stress by interacting with ZmbHLH161 and ZmA0A1D6GLB9
作者：Zhang, Shipeng;Lu, Yuncai;Zhang, Shipeng;Zheng, Dengyu;Gao, Yuqi;She, Meng;Wu, Zhongyi;Zhang, Zhongbao;Gao, Yuqi
关键词：Maize; ZmJAZ13; Drought stress; Salt stress; Response to adversity
-
Molecular Elucidation of Anthocyanin Accumulation Mechanisms in Hippeastrum hybridum Cultivars
作者：Guo, Pengyu;Xing, Chuanji;Ye, Jiacheng;Xue, Jing;Zhang, Xiuhai;Chen, Xuqing;Guo, Pengyu;Hu, Zongli;Chen, Guoping;Ye, Jiacheng;Mur, Luis A. J.;Di, Bao
关键词：
Hippeastrum ; petals; anthocyanins; transcriptomics; metabolomics; WGCNA; Y2H -
The NAC transcription factor LpNAC48 promotes trichome formation in Lilium pumilum
作者：Xin, Yin;Pan, Wenqiang;Wu, Jingxiang;Zhang, Mingfang;Ma, Yang;Zhang, Xiuhai;Du, Yunpeng;Xin, Yin;Pan, Wenqiang;Zhao, Yajie;Li, Jingru;Wang, Shaokun;Wu, Jingxiang;Shi, Jinxin;Ma, Yang;Wu, Jian;Pan, Wenqiang;Zhang, Xiuhai;Du, Yunpeng;Yang, Chenglong;Fang, Shaozhong;Liang, Yuwei;Zaccai, Michele
关键词：
-
A natural variation of flavone synthase II gene enhances flavone accumulation and confers drought adaptation in chrysanthemum
作者：Luo, Jiayi;Han, Mingzheng;Song, Zhenzhen;Zhang, Haixia;Zhao, Yafei;Ma, Chao;Luo, Chang;Huang, Conglin;Wang, Qinrui;Gao, Qiang;Lin, Tao
关键词：Chrysanthemum; drought; flavone; FNSII; GWAS
