The effect of calcium chloride injection on shear force and caspase activities in bovine longissimus muscles during postmortem conditioning
文献类型： 外文期刊
作者： Cao, J. 1 ; Yu, X. 3 ; Khan, M. A. 2 ; Shao, J. 2 ; Xiang, Y. 4 ; Zhou, G. 2 ;
作者机构： 1.Ningbo Univ, Coll Life Sci & Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
2.Nanjing Agr Univ, Dept Food Sci, Minist Sci & Technol, Natl Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China
3.Henan Inst Sci & Technol, Coll Food Sci & Technol, Xinxiang 453003, Henan, Peoples R China
4.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang 550008, Peoples R China
关键词： calcium chloride;caspases;conditioning;proteolysis;tenderness
期刊名称：ANIMAL （ 影响因子：3.24； 五年影响因子：3.223 ）
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收录情况： SCI
摘要： Tenderness is considered as the most important quality determinant of meat. Calcium chloride application has been shown to improve tenderness by regulating endogenous proteinases. This study was designed to determine the effect of 300 mM calcium chloride injection on myofibrillar structures, caspase activities and shear force in longissimus muscles of bulls during postmortem storage of 7 days. Myofibrillar fragmentation index was determined as an index of proteolysis occurring in muscle fibers and associated proteins. Maximum tenderness was observed at days 4 and 7 in both treated and control samples. The injection of calcium chloride significantly increased myofibrillar proteolysis and improved tenderness at postmortem days 4 and 7. The treatment reduced caspase-9 activity at 4 h and day 4, whereas those of caspase-8 and -3 activities at days 1 and 4 with respect to control. The improved tenderness and increased myofibril fragmentation with decreased caspase activities suggested that the proteolytic systems activated with calcium chloride injection possibly behave independent of the caspase system.
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