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Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis

文献类型： 外文期刊

作者： Zhang, Bo 1 ; Wang, Xianli 1 ; Sha, Zhenxia 1 ; Yang, Changgeng 1 ; Liu, Shanshan 1 ; Wang, Na 1 ; Chen, Song-Lin 1 ;

作者机构： 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Cynoglossus semilaevis;Cyto-pathic effects;Gonadal cell line;Half-smooth tongue sole;Karyotype;Virus susceptibility

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES （ 影响因子：6.58； 五年影响因子：6.478 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole (Cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called CSGC from a juvenile gonad of this organism. CSGC is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the heterogametic W chromosome, lacks a female specific marker and expresses the dmrt, a marker for testicular somatic cells. Therefore, CSGC appears to consist of testicular somatic cell cells. We show that this cell line is effective for infection by the turbot reddish body iridovirus and flounder lymphocystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electon microscopy, demonstrateing that CSGC is suitable to study interactions between virus and host cells. As a first fish testicular somatic cell line of the ZZ-ZW genetic sex determination system, CSGC will be a useful tool to study sex-related events and interactions between somatic cells and germ cells during spermatogenesis.

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[1]Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis). Zheng, Yuan,Wang, Na,Xie, Ming-Shu,Sha, Zhen-Xia,Chen, Song-Lin,Zheng, Yuan,Xie, Ming-Shu.

[2]Establishment, characterization and virus susceptibility of a kidney-derived cell line from southern flounder, Paralichthys lethostigma Jordan & Gilbert. Wang, N.,Wang, X-L,Sha, Z-X,Tian, Y-S,Chen, S-L,Chen, S-L. 2011

[3]Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus). Wang, N.,Wang, X. L.,Sha, Z. X.,Tian, Y. S.,Chen, S. L..

[4]Induction of Mitogynogenetic Diploids and Identification of WW Super-female Using Sex-Specific SSR Markers in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Chen, Song-Lin,Ji, Xiang-Shan,Shao, Chang-Wei,Li, Wen-Long,Yang, Jing-Feng,Liang, Zuo,Liao, Xiao-Lin,Xu, Gen-Bo,Xu, Ying,Song, Wen-Tao,Ji, Xiang-Shan. 2012

[5]Growth differences and differential expression analysis of pituitary adenylate cyclase activating polypeptide (PACAP) and growth hormone-releasing hormone (GHRH) between the sexes in half-smooth tongue sole Cynoglossus semilaevis. Ji, Xiang-Shan,Chen, Song-Lin,Xu, Tian-Jun,Yang, Jing-Feng,Tian, Yong-Sheng,Ji, Xiang-Shan,Jiang, Yun-Liang. 2011

[6]Molecular cloning, characterization and expression analysis of Sox9a and Foxl2 genes in half-smooth tongue sole (Cynoglossussemilaevis). Dong Xiaoli,Chen Songlin,Shao Changwei,Dong Xiaoli,Ji Xiangshan. 2011

[7]A first generation BAC-based physical map of the half-smooth tongue sole (Cynoglossus semilaevis) genome. Zhang, Junjie,Shao, Changwei,Zhang, Liyan,Liu, Kun,Gao, Fengtao,Dong, Zhongdian,Chen, Songlin,Zhang, Junjie,Zhang, Junjie,Xu, Peng. 2014

[8]Application of AFLP markers for population genetic study on half-smooth tongue sole Cynoglossus semilaevis. Liu Yunguo,Liu Yunguo,Li Junfeng,Ye Naihao. 2011

[9]Molecular and phenotypic characterization of Vibrio aestuarianus, a pathogen of the cultured tongue sole, Cynoglossus semilaevis Gunther. Zhang, X-J,Qin, G-M,Yan, B-L,Liang, L-G,Bing, X-W. 2011

[10]Comparison of chromosome preparation methods for the different developmental stages of the half-smooth tongue sole, Cynoglossus semilaevis. Shao, Chang-Wei,Wu, Peng-Fei,Wang, Xian-Li,Tian, Yong-Sheng,Chen, Song-Lin. 2010

[11]Construction of a microsatellite-based genetic linkage map for half-smooth tongue sole Cynoglossus semilaevis. Song, Wentao,Miao, Guidong,Zhao, Yongwei,Niu, Yuze,Pang, Renyi,Liao, Xiaolin,Shao, Changwei,Chen, Songlin,Song, Wentao. 2013

[12]Isolation of Female-Specific AFLP Markers and Molecular Identification of Genetic Sex in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Chen, Song-Lin,Li, Jing,Deng, Si-Ping,Tian, Yong-Sheng,Wang, Qing-Yin,Zhuang, Zhi-Meng,Sha, Zhen-Xia,Xu, Jian-Yong.

[13]Construction of Two BAC Libraries from Half-Smooth Tongue Sole Cynoglossus semilaevis and Identification of Clones Containing Candidate Sex-Determination Genes. Shao, Chang-Wei,Chen, Song-Lin,Xu, Jian-Yong,Sha, Zhen-Xia,Dong, Xiao-Li,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin.

[14]C-banding pattern and nucleolar organizer regions of Cynoglossus semilaevis Gunther, 1873. Wu Di,Zhang Shicui,Zhuang Zhimeng,Pang Qiuxiang,Wang Changliu,Wan Ruijing.

[15]Initiation of two ovarian cell lines from Fugu rubripes (Temminck et. Schlegel). Zheng Debin,Pan Luqing,Zhang Bo,Song Wenping,Ma Chao,Xiao Guangxia. 2015

[16]Molecular cloning, genomic structure and expression analysis of major histocompatibility complex class I alpha gene of half-smooth tongue sole (Cynoglossus semilaevis). Chen, Song-lin,Xu, Tian-jun. 2011

[17]Genomic structure, polymorphism and expression analysis of growth hormone-releasing hormone and pituitary adenylate cyclase activating polypeptide genes in the half-smooth tongue sole (Cynoglossus semilaevis). Ma, Q.,Liu, S. F.,Zhuang, Z. M.,Lin, L.,Sun, Z. Z.,Liu, C. L.,Tang, Q. S.,Ma, Q.,Lin, L.,Su, Y. Q.. 2011

[18]Characterization of the cyp19a1a gene from a BAC sequence in half-smooth tongue sole (Cynoglossus semilaevis) and analysis of its conservation among teleosts. Shao Changwei,Shao Changwei,Liu Shanshan,Liu Changlin,Chen Songlin,Liu Geng. 2013

[19]Unexpected variations Of beta(2)-microglobulin gene in the half-smooth tongue sole. Xu, Tian-jun,Sha, Zhen-xia,Chen, Song-lin,Xu, Tian-jun. 2010

[20]Molecular characterization and expression analysis of Patched 1 gene in the half-smooth tongue sole (Cynoglossus semilaevis). Dong Zhongdian,Qi Qian,Zhang Ning,Wen Haishen,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin,Dong Zhongdian,Qi Qian,Zhang Ning,Shao Changwei,Zhang Liyan,Chen Songlin. 2016

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