Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis
文献类型: 外文期刊
作者: Zhang, Bo 1 ; Wang, Xianli 1 ; Sha, Zhenxia 1 ; Yang, Changgeng 1 ; Liu, Shanshan 1 ; Wang, Na 1 ; Chen, Song-Lin 1 ;
作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
关键词: Cynoglossus semilaevis;Cyto-pathic effects;Gonadal cell line;Half-smooth tongue sole;Karyotype;Virus susceptibility
期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES ( 影响因子:6.58; 五年影响因子:6.478 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Spermatogenesis within the adult testis is an excellent system for studying stem cell renewal and differentiation, which is under the control of testicular somatic cells. In order to understanding spermatogenesis in the half-smooth tongue sole (Cynoglossus semilaevis) as a marine fish model of aquaculture importance, we established a cell line called CSGC from a juvenile gonad of this organism. CSGC is composed of fibroblast-like cells, retains a diploid karyotype of 42 chromosomes, lacks the heterogametic W chromosome, lacks a female specific marker and expresses the dmrt, a marker for testicular somatic cells. Therefore, CSGC appears to consist of testicular somatic cell cells. We show that this cell line is effective for infection by the turbot reddish body iridovirus and flounder lymphocystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electon microscopy, demonstrateing that CSGC is suitable to study interactions between virus and host cells. As a first fish testicular somatic cell line of the ZZ-ZW genetic sex determination system, CSGC will be a useful tool to study sex-related events and interactions between somatic cells and germ cells during spermatogenesis.
- 相关文献
作者其他论文 更多>>
-
Decoding the fish genome opens a new era in important trait research and molecular breeding in China
作者:Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Zhou, Qian;Wang, Jialin;Chen, Zhangfan;Wang, Na;Li, Ming;Wang, Lei;Si, Yufeng;Lu, Sheng;Cui, Zhongkai;Liu, Xuhui;Chen, Songlin;Li, Jiongtang
关键词:fish genomics; economic traits; genomic selection; genome editing; genomic breeding
-
Comparative analysis of the structural and compositional change of spotted sea bass (Lateolabrax maculatus) gut microflora following Aeromonas veronii infection and the effects of Lactobacillus plantarum on these changes
作者:Lin, Changhong;Lin, Changhong;Lin, Changhong;Zhang, Bo;Zhang, Boa;Qiu, Lihua;Zhang, Bo;Wang, Pengfei;Zhang, Boa;Yan, Lulu;Zhao, Chao;Qiu, Lihua;Zhang, Bo;Wang, Pengfei;Zhang, Boa;Yan, Lulu;Zhao, Chao;Qiu, Lihua;Zhang, Bo;Wang, Pengfei;Zhang, Boa;Zhao, Chao;Zhao, Chao
关键词:Lateolabrax maculatus; Aeromonas veronii; Gut microbiota; intestinal flora; pathogen.
-
Rhodiola rosea L. improved intestinal digestive enzyme activities, inflammatory response, barrier and microbiota dysbiosis in Lateolabrax maculatus juveniles fed with high-carbohydrate diets
作者:Zheng, Luzhe;Wang, Zhanzhan;Zhang, Bo;Zhao, Chao;Qiu, Lihua;Zhou, Chuanpeng;Zheng, Luzhe;Zhao, Chao;Yan, Lulu;Wang, Pengfei;Wang, Jun;Wang, Yun;Zhou, Chuanpeng;Yan, Lulu;Wang, Pengfei;Wang, Jun;Wang, Yun;Lin, Heizhao;Zhou, Chuanpeng;Lin, Heizhao
关键词:Intestinal barrier; Intestinal immunity; Inflammatory; Intestinal microbiota; Rhodiola rosea L.; Lateolabrax maculatus
-
Characterization and immune role of class B scavenger receptor member 1 in spotted sea bass (Lateolabrax maculatus)
作者:Fu, Yichen;Lin, Changhong;Fu, Yichen;Zhao, Chao;Lin, Changhong;Zhang, Bo;Yan, Lulu;Wang, Pengfei;Qiu, Lihua;Zhao, Chao;Zhang, Bo;Yan, Lulu;Wang, Pengfei;Qiu, Lihua;Qiu, Lihua
关键词:Lateolabrax maculatus; SRB1; Aeromonas veronii; Immune response
-
Single-cell transcriptome landscape of zebrafish liver reveals hepatocytes and immune cell interactions in understanding nonalcoholic fatty liver disease
作者:Huang, Yingyi;Liu, Xiang;Wang, Hong-Yan;Li, Yubang;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Huang, Yingyi;Liu, Xiang;Wang, Hong-Yan;Li, Yubang;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Chen, Jian-Yang;Zhang, Xianghui;Lu, Yifang;Wang, Qian;Fan, Guangyi;Chen, Jian-Yang;Zhang, Xianghui;Lu, Yifang;Fan, Guangyi;Dong, Zhongdian;Wang, Zhongduo;Liu, Shanshan;Fan, Guangyi;Liu, Shanshan;Zou, Jun
关键词:
-
Three Copies of zbed1 Specific in Chromosome W Are Essential for Female-Biased Sexual Size Dimorphism in Cynoglossus semilaevis
作者:Sun, Yuqi;Sun, Yuqi;Li, Xihong;Mai, Jiaqi;Xu, Wenteng;Wang, Jiacheng;Zhang, Qi;Wang, Na;Mai, Jiaqi;Wang, Jiacheng;Zhang, Qi
关键词:Cynoglossus semilaevis; sexual size dimorphism; zbed1
-
Study on the mechanism of miR-7562 regulating ATG5 and ATG12 genes in Penaeus monodon under Vibrio harveyi infection
作者:Zhao, Chao;Wang, Chunlin;Zhao, Chao;Lin, Changhong;Zhang, Bo;Wang, Pengfei;Zhang, Bo;Yan, Lulu;Qiu, Lihua;Zhao, Chao;Zhang, Bo;Wang, Pengfei;Zhang, Bo;Yan, Lulu;Qiu, Lihua;Zhao, Chao;Wang, Pengfei;Qiu, Lihua;Lin, Changhong
关键词:Penaeus monodon; microRNA; Autophagy-related gene; Autophagy; Vibrio harveyi