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Isolation and characterization of a FLOWERING LOCUS T homolog from pineapple (Ananas comosus (L.) Merr)

文献类型： 外文期刊

作者： Lv, LingLing 2 ; Duan, Jun 1 ; Xie, JiangHui 2 ; Wei, ChangBin 2 ; Liu, Yuge 2 ; Liu, ShengHui 2 ; Sun, GuangMing 2 ;

作者机构： 1.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, S Subtrop Crops Res Inst, Zhanjiang 524091, Guangdong, Peoples R China

关键词： Characterization;Flower development;FT;Isolation;Pineapple;Transformation

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： FLOWERING LOCUS T (FT)-like genes are crucial regulators of flowering in angiosperms. A homolog of FT, designated as AcFT (GenBank ID: HQ343233), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcFT is 915. bp in length and contains an ORF of 534. bp, which encodes a protein of 177 aa. Molecular weight was 19.9. kDa and isoelectric point was 6.96. The deduced protein sequence of AcFT was 84% and 82% identical to homologs encoded by CgFT in Cymbidium goeringii and OgFT in Oncidium Gower Ramsey respectively. Quantitative real-time PCR (qRT-PCR) analyses showed that the expression of AcFT was high in flesh and none in leaves. qRT-PCR analyses in different stages indicated that the expression of AcFT reached the highest level on 40 d after flower inducing, when the multiple fruit and floral organs were forming. The 35S::. AcFT transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.

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