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Development of a female-specific RAPD marker for Sargassum thunbergii gender identification using bulked segregant analysis

文献类型： 外文期刊

作者： Liu, Fuli 1 ; Sun, Xiutao 1 ; Wang, Wenjun 1 ; Liang, Zhourui 1 ; Wang, Feijiu 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Sargassum thunbergii;Random amplified polymorphism DNA;Gender identification;Bulked segregant analysis

期刊名称：AQUATIC BOTANY （ 影响因子：2.473； 五年影响因子：2.417 ）

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收录情况： SCI

摘要： In the present paper, we report the development of a female-specific Random Amplified Polymorphism DNA (RAPD) marker for Sargassum thunbergii gender identification using bulked segregant analysis. Five male and five female mature individuals were selected as the samples, from which the genomic DNA were extracted and mixed to produce the male DNA pool and the female DNA pool respectively. The two DNA pools were subjected to RAPD analysis using a total of 100 decamer primers. Out of the 617 RAPD markers only one marker named F289 was polymorphic between the two DNA pools (only appeared in female DNA pool). Marker F289 was also only present in the five female individuals but absent in the five male individuals who made up the female and male DNA pool respectively. The female-specific character of marker F289 was further verified in the 12 male and 12 female individuals sampled randomly from the field. These results indicated that the marker F289 was a female-specific marker, which may link to the sex determination in S. thunbergii. Consequently, we concluded that F289 can be used as a reliable molecular marker for S. thunbergii gender identification. This marker will facilitate the artificial seedlings rearing based on sexual reproduction and also can be utilized in population genetics study as well as in sex-determining mechanism study for S. thunbergii

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