Isolation and expression of a cold-responsive gene PtCBF in Poncirus trifoliata and isolation of citrus CBF promoters
文献类型： 外文期刊
作者： He, L. G. 1 ; Wang, H. L. 1 ; Liu, D. C. 1 ; Zhao, Y. J. 1 ; Xu, M. 2 ; Zhu, M. 1 ; Wei, G. Q. 1 ; Sun, Z. H. 1 ;
作者机构： 1.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China
2.Hubei Acad Agr Sci, Inst Fruit & Tea, Wuhan 430209, Peoples R China
关键词： abscisic acid;Citrus grandis;Citrus jambhiri;Citrus sinensis;low temperature;trifoliate orange
期刊名称：BIOLOGIA PLANTARUM （ 影响因子：1.747； 五年影响因子：2.146 ）
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收录情况： SCI
摘要： C-repeat/dehydration-responsive element binding factor (CBF) plays important roles in cold response network in plants. Here, one member of CBF coding gene family in trifoliate orange (Poncirus trifoliata), designated as PtCBF, was isolated. Semi-quantitative reverse transcription-polymerase chain reactions showed up-regulation of PtCBF not only under low temperature but also induced by abscisic acid. Additionally, the CBF genomic fragments in four citrus species including trifoliate orange, sweet orange (Citrus sinensis), pummel (Citrus grandis) and rough lemon (Citrus jambhiri) were isolated with complete open reading frames. According to the results of alignment analysis between full length cDNA and genomic DNA sequences in trifoliate orange, there were no introns in PtCBF. Moreover, the results of multiple sequence alignment analysis and phylogenetic analysis on putative protein sequences suggested that the AP2 DNA binding domains and CBF signature sequences were highly conserved in four citrus CBF proteins. Finally, the CBF promoters in above citrus species were isolated, which provides some information concerning promoter function.
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