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Polyethylene glycol-mediated transformation of fused egfp-hph gene under the control of gpd promoter in Pleurotus eryngii

文献类型： 外文期刊

作者： Yin, Yonggang 1 ; Liu, Yu 1 ; Jin, Haojie 3 ; Wang, Shouxian 1 ; Zhao, Shuang 1 ; Geng, Xiaoli 1 ; Li, Ming 2 ; Xu, Feng 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

2.Agr Univ Hebei, Coll Hort, Baoding 071001, Hebei, Peoples R China

3.Univ Aarhus, Dept Mol Biol & Genet, DK-8000 Aarhus, Denmark

关键词： Pleurotus eryngii;Polyethylene glycol;Promoter;Protoplasts;Transformation efficiency

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

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收录情况： SCI

摘要： Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the control of the strong and constitutive native gpd promoter from P. eryngii. The recombinant plasmid was used to transform of P. eryngii protoplasts. Successful transformation was demonstrated by molecular analyses. Moreover, the mycelia of the transformants showed green epipolic dispersion on fluorescence microscopy. About 90-210 transformants were produced per mug plasmid DNA per 10~7 viable protoplasts.

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