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Genetic variability of the Asian arowana, Scleropages formosus, based on mitochondrial DNA genes

文献类型: 外文期刊

作者: Mu, Xi-dong 1 ; Song, Hong-mei 1 ; Wang, Xue-jie 1 ; Yang, Ye-xin 1 ; Luo, Du 1 ; Gu, Dang-en 1 ; Luo, Jian-ren 2 ; Hu, Y 1 ;

作者机构: 1.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

2.Chinese Acad Fishery Sci, Ke

关键词: Scleropages formosus;Mitochondrial DNA;Genetic variability;Relationships

期刊名称:BIOCHEMICAL SYSTEMATICS AND ECOLOGY ( 影响因子:1.381; 五年影响因子:1.351 )

ISSN:

年卷期:

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收录情况: SCI

摘要: The present study examined the genetic variation in the mitochondrial 12S rRNA, 16S rRNA and cytochrome c oxidase subunit 3 (cox3) genes among three varieties of Scleropages formosus, and their phylogenetic relationships were constructed. Among all the examined isolates of S. formosus, sequence variation ranged from 0 to 1.66% for 12S, from 0 to 2.4% for 165 and from 0 to 3.2% for pcox3. AMOVA analysis of the three mtDNA genes showed that the majority of the total molecular variation occurred among varieties within the groups (55.6%) with 48.4% of variation within the varieties. Additionally, a significant genetic differentiation (P < 0.05) was revealed within S. formosus. Phylogenetic analysis based on the combined sequences showed that all S. formosus clustered together and effectively grouped the green arowanas together. These results demonstrate that the three mtDNA sequences are polymorphic but are not suitable markers for comparing the genetic relationships among the different varieties of S. formosus

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