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Rapid and sensitive determination of five amine biomarkers in plasma samples from stroke patients by MEKC with precolumn derivatization

文献类型： 外文期刊

作者： Peng, Ling 1 ; Wang, Na 2 ; Si, Hui 2 ; Wu, Caixia 3 ; Zhang, Xiaoling 4 ; Yang, Qiao 4 ;

作者机构： 1.Zhengzhou Univ, Div Pharm, Affiliated Hosp 2, Zhengzhou 450014, Peoples R China

2.China Tobacco Hubei Ind Co Ltd, Ctr Technol, Wuhan 430040, Peoples R China

3.Henan Ctr Dis Control & Prevent, Zhengzhou 450016, Peoples R China

4.Chinese Acad Fishery Sci, E China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

关键词： 3-(2-Furoyl)quinoline-2- carboxaldehyde;Amine biomarker;MEKC;Plasma samples;Precolumn derivatization;Stroke

期刊名称：CHROMATOGRAPHIA （ 影响因子：2.044； 五年影响因子：1.725 ）

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收录情况： SCI

摘要： The current diagnosis of stroke remains hampered and delayed due to lack of a suitable mechanism for the rapid, accurate, and analytically sensitive biomarkerbased testing methods. In the present study, a MEKC method coupled with highly sensitive laser-induced fluorescence detection and precolumn derivatization was originally described for the rapid, sensitive determination of five amine biomarkers including homocysteine (Hcy), glutamic acid (Glu), putrescine (Put), spermine (Spm) and spermidine (Spd) of stroke in plasma samples from stroke patients. 3-(2-Furoyl)quinoline-2-carboxaldehyde (FQ) was selected as the fluorescence reagent and optimized for the advantageous high-throughput derivatization with total 30 lL reaction system in 384-well microplates for the rapid determination of the targeted analytes. Results showed that the proposed derivatization reaction approach coupled with MEKC-LIF was an efficient method for the rapid determination of five amine biomarkers of stroke in plasma samples.

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