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Cloning, characterization and expression of a novel lipase gene from marine psychrotrophic Yarrowia lipolytica

文献类型： 外文期刊

作者： Sheng, Jun 1 ; Wang, Fang 1 ; Wang, HaiYing 1 ; Sun, Mi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Lab Enzyme Engn, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： Recombinant lipase;Expression;Characterization;Psychrotrophic Yarrowia lipolytica

期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）

ISSN：

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收录情况： SCI

摘要： A marine Yarrowia lipolytica yeast, named Bohaisea-9145, was found to secrete a large amount of lipase into the medium. A gene coding for lipase was cloned from the genome of this strain and expressed successfully in Escherichia coli BL21 (DE3). A maximum activity of 17.6 U/mg was obtained from cellular extract of E. coli harboring the lipase gene. The recombinant lipase exhibits one band with a molecular mass of about 44 kDa on SDS-PAGE. The optimal temperature and pH of the purified lipase were 35°C and 8.5, respectively. The K_m and V_(max) values of the lipase for p-nitrophenyl laurate were 0.582 μM and 0.124 mmol min~(?1) mg~(?1) under 35°C,respectively. Additionally, the purified lipase showed a high activity and stability over a wide range of temperatures,especially in the low and moderate temperatures, suggesting its potential for industrial applications.

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