Analysis of the immune response in infections of the goldfish (Carassius auratus L.) with Mycobacterium marinum
文献类型： 外文期刊
作者： Hodgkinson, Jordan W. 1 ; Ge, Jun-Qing 1 ; Grayfer, Leon 1 ; Stafford, James 1 ; Belosevic, Miodrag 1 ;
作者机构： 1.Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada
2.Univ Alberta, Sch Publ Hlth, Edmonton, AB T6G 2E9, Canada
3.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou, Fijian, Peoples R China
关键词： Cytokine receptors;Cytokines;Fish;INOS;Macrophage;Mycobacteria;NADPH;Nitric oxide;Respiratory burst
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： The rapid doubling time and genetic relatedness of the fish pathogen Mycobacterium marinum to Mycobacterium tuberculosis has rendered the former an attractive model for investigating mycobacterial host-pathogen interactions. We employed the M. marinum-goldfish infection model to investigate the in vivo immune responses to this pathogen in the context of a natural host. Histological analysis revealed mycobacterial infiltrates in goldfish kidney and spleen tissues, peaking 28. days post infections (dpi). Quantitative gene expression analysis showed significant increases of mRNA levels of pro-inflammatory cytokines (IFNγ, IL-12p40, IL-1β1) and cytokine receptors (IFNGR1-1, TNFR2) at 7. dpi. Conversely, the gene expression levels of key anti-inflammatory cytokines TGFβ and IL-10 were elevated at 14. dpi. Furthermore, M. marinum infections markedly increased the cytokine-primed oxidative burst responses of isolated kidney phagocytes at 7 but not 56. dpi. We believe that the M. marinum-goldfish infection model will be invaluable in furthering the understanding of the mycobacterium host-pathogen interface.
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